Rabbit MADD (CT) Antibody | anti-MADD antibody

Anti-MADD Antibody

Cat. Num. AAA194115

• Gene Names: MADD; DENN; IG20; RAB3GEP; FLJ35600; FLJ36300; KIAA0358
• Applications: Immunoblot, Western Blot
• Synonyms: MADD (CT); Anti-MADD Antibody; Anti-MADD (CT); MADD; anti-MADD antibody

• Host: Rabbit
• Specificity: Anti-MADD (CT) [Rabbit antibody to peptide corresponding to aa 1570-1588 of human MADD. Recognizes human and mouse MADD.]; This antibody recognizes human and mouse MADD (200-220 kD).
• Form/Format: 100 ug IgG in PBS, pH 7.4, purified by immunoaffinity chroma-tography.

• Applicable Applications for anti-MADD antibody: Immunoblot
• Application Notes: Immunoblotting : use at 1:250-1:500 dilution.; Positive control: Whole cell lysate from HeLa or NIH3T3 cells.
• Antigen: Peptide corresponding to aa 1570-1588 of human MADD. This peptide sequence is identical to that of DENN and differs by one amino acid with rat GDP/GTP exchange protein RAB3-GEP.
• Stabilizer: None
• Preservatives: 0.02% sodium azide.
• Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.

Related Product Information for anti-MADD antibody

MAP kinase-activating death domain protein (MADD) was initially identified as the type 1 tumor necrosis factor receptor (TNFR1). Overexpression of MADD activates MAP kinases ERK and JNK and induces the phosphorylation of cytosolic phospholipase A2. MADD shares 98% homology with DENN (differentially expressed in neoplasic vs. normal cells) which was recently identified as a substrate for c-jun N-terminal kinase 3 (JNK3). MADD has greather than 94% homology with a GDO/GTP exchange protein , Rab3-GEP, and it is 87% homologous with KIAA0358, a brain protein of unknown function. Identification of MADD as a component of the TNFR1 signalling complex and the similarity between MADD and Rab3-GEP provides a connection between TNFR1 activation and downstream MAP kinase activity through a guanine-nucleotide exchange protein.

Product Categories/Family for anti-MADD antibody

Apoptosis Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 209863002
• NCBI GeneID: 8567
• NCBI Accession #: NP_001129416.1
• NCBI GenBank Nucleotide #: NM_001135944.1
• UniProt Accession #: Q7KYV8; Q8WXG6
• Molecular Weight: 5,180 Da
• NCBI Official Full Name: MAP kinase-activating death domain protein isoform j
• NCBI Official Synonym Full Names: MAP-kinase activating death domain
• NCBI Official Symbol: MADD
• NCBI Official Synonym Symbols: DENN; IG20; RAB3GEP; FLJ35600; FLJ36300; KIAA0358
• NCBI Protein Information: MAP kinase-activating death domain protein; OTTHUMP00000197131; OTTHUMP00000197132; OTTHUMP00000197133; OTTHUMP00000197134; OTTHUMP00000197135; OTTHUMP00000197136; OTTHUMP00000197137; OTTHUMP00000237191; OTTHUMP00000238029; Rab3 GDP/GTP exchange factor; insulinoma glucagonoma clone 20; differentially expressed in normal and neoplastic cells
• UniProt Protein Name: DENN protein
• Protein Family: Malonyl-S-ACP:biotin-protein carboxyltransferase
• UniProt Gene Name: DENN
• UniProt Entry Name: Q7KYV8_HUMAN

NCBI Description

Tumor necrosis factor alpha (TNF-alpha) is a signaling molecule that interacts with one of two receptors on cells targeted for apoptosis. The apoptotic signal is transduced inside these cells by cytoplasmic adaptor proteins. The protein encoded by this gene is a death domain-containing adaptor protein that interacts with the death domain of TNF-alpha receptor 1 to activate mitogen-activated protein kinase (MAPK) and propagate the apoptotic signal. It is membrane-bound and expressed at a higher level in neoplastic cells than in normal cells. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Uniprot Description

MADD: a guanine nucleotide exchange factor (GEF) that activates RAB3A/C/D. A GEF for Rab27A/B in melanocytes and during isoproterenol (IPR)-stimulated amylase release from rat parotid acinar cells. Plays a significant role in regulating cell proliferation, survival and death through alternatively spliced isoforms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis). Interacts with the death domain of TNFRSF1A through its own death domain. Highly expressed in fetal brain and kidney, adult testis, ovary, brain and heart. Overexpression of MADD activates the mitogen-activated protein (MAP) kinase extracellular signal-regulated kinase (ERK). Expression of the MADD death domain stimulates both the ERK and c-JUN N-terminal kinase MAP kinases and induces the phosphorylation of cytosolic phospholipase A2. Isoform 1 is pro-apoptotic, isoform 5 is anti-apoptotic and isoform 3 and isoform 4 have no effect. Isoform 5 is constitutively expressed in all tissues. Isoform 7 is expressed in fetal liver and in several cancer cell lines. Isoform 5 is associated with increased cell proliferation and isoform 2 with decreased proliferation. Belongs to the MADD family. Seven isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; GEFs; GEFs, Rab; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: cytoplasm; integral to membrane; plasma membrane

Molecular Function: protein binding; death receptor binding; Rab guanyl-nucleotide exchange factor activity; protein kinase activator activity

Biological Process: regulation of apoptosis; regulation of Rab protein signal transduction; cell surface receptor linked signal transduction; regulation of cell cycle; activation of MAPK activity