Typical Testing Data/Standard Curve (for reference only)
Rat B-cell CLL/lymphoma 2 ELISA Kit | BCL2 elisa kit
Rat B-cell CLL/lymphoma 2, BCL2 ELISA Kit
Gene Names
Bcl2; Bcl-2
Reactivity
Rat
Synonyms
B-cell CLL/lymphoma 2; Rat B-cell CLL/lymphoma 2; BCL2 ELISA Kit; Rat B-cell CLL/lymphoma 2 (BCL2) ELISA kit; Bcl-2; B-cell lymphoma protein 2; OTTHUMP00000163680; BCL2 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat BCL2. No significant cross-reactivity or interference between rat BCL2 and analogues was observed.
Sequence Length
236
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
0.312 ng/ml -20 ng/ml.
Sensitivity
The minimum detectable dose of rat BCL2 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for BCL2 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BCL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BCL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BCL2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BCL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,622 Da
NCBI Official Full Name
apoptosis regulator Bcl-2
NCBI Official Synonym Full Names
B-cell CLL/lymphoma 2
NCBI Official Symbol
Bcl2
NCBI Official Synonym Symbols
Bcl-2
NCBI Protein Information
apoptosis regulator Bcl-2; Bcl2-like protein; B-cell leukemia/lymphoma 2; B cell lymphoma 2 associated oncogene
UniProt Protein Name
Apoptosis regulator Bcl-2
Protein Family
UniProt Gene Name
Bcl2
UniProt Synonym Gene Names
Bcl-2
UniProt Entry Name
BCL2_RAT
NCBI Description
an anti-apoptotic protein; involved in inhibiting cell death in many different cell types [RGD, Feb 2006]
Uniprot Description
Function: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Research Articles on BCL2
Similar Products
Product Notes
The Rat BCL2 bcl2 (Catalog #AAA704498) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA704498 ELISA Kit recognizes Rat BCL2. It is sometimes possible for the material contained within the vial of "B-cell CLL/lymphoma 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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