Western Blot (WB)
(Western blotAll lanes: Glyceraldehyde-3-phosphate dehydrogenase antibody at 2ug/mlLane 1: Hale whole cell lysateLane 2: A549 whole cell lysateLane 3: HepG2 whole cell lysateLane 4: K562 whole cell lysateLane 5: Jurkats whole cell lysateLane 6: MDA-MB-231 whole cell lysateLane 7: LO2 whole cell lysateLane 8: U251 whole cell lysateLane 9: MCF-7 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 37,32 kDaObserved band size: 37 kDa)
Rabbit anti-Human Glyceraldehyde-3-phosphate dehydrogenase Polyclonal Antibody | anti-GAPDH antibody
Rabbit anti-human Glyceraldehyde-3-phosphate dehydrogenase polyclonal Antibody
Western Blot (WB)
(Western blotAll lanes: Glyceraldehyde-3-phosphate dehydrogenase antibody at 2ug/mlLane 1: Hale whole cell lysateLane 2: A549 whole cell lysateLane 3: HepG2 whole cell lysateLane 4: K562 whole cell lysateLane 5: Jurkats whole cell lysateLane 6: MDA-MB-231 whole cell lysateLane 7: LO2 whole cell lysateLane 8: U251 whole cell lysateLane 9: MCF-7 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 37,32 kDaObserved band size: 37 kDa)
Western Blot (WB)
(Western blotAll lanes: Glyceraldehyde-3-phosphate dehydrogenase antibody at 2ug/mlLane 1: Hale whole cell lysateLane 2: A549 whole cell lysateLane 3: HepG2 whole cell lysateLane 4: K562 whole cell lysateLane 5: Jurkats whole cell lysateLane 6: MDA-MB-231 whole cell lysateLane 7: LO2 whole cell lysateLane 8: U251 whole cell lysateLane 9: MCF-7 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 37,32 kDaObserved band size: 37 kDa)
Western Blot (WB)
(Western blotAll lanes: GAPDH antibody at 2ug/mlLane 1: Hela whole cell lysateLane 2: 293T whole cell lysateLane 3: Jurkat whole cell lysateLane 4: Mouse kidney tissueLane 5: Rat muscle tissueLane 6: Rat lung tissueLane 7: Zebrafish lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 37,32 kDaObserved band size: 37 kDa)
Western Blot (WB)
(Western blotAll lanes: GAPDH antibody at 2ug/mlLane 1: Hela whole cell lysateLane 2: 293T whole cell lysateLane 3: Jurkat whole cell lysateLane 4: Mouse kidney tissueLane 5: Rat muscle tissueLane 6: Rat lung tissueLane 7: Zebrafish lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 37,32 kDaObserved band size: 37 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in: hepG2 cell,mouse brain,mouse skeletal muscleAll lanes: GAPDH antibody at 2.6ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 37,32 kDaObserved band size: 37 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in: hepG2 cell,mouse brain,mouse skeletal muscleAll lanes: GAPDH antibody at 2.6ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 37,32 kDaObserved band size: 37 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in:Hela whole cell lysate,A549 whole cell lysate,HEK293 whole cell lysate,HepG2 whole cell lysate,Jurkat whole cell lysate,K562 whole cell lysateAll lanes: GAPDH antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 37,32 kDaObserved band size: 37 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in:Hela whole cell lysate,A549 whole cell lysate,HEK293 whole cell lysate,HepG2 whole cell lysate,Jurkat whole cell lysate,K562 whole cell lysateAll lanes: GAPDH antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 37,32 kDaObserved band size: 37 kDa)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse liver using MBS969811 at dilution 1:50)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse liver using MBS969811 at dilution 1:50)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human prostate cancer using MBS969811 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human prostate cancer using MBS969811 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human pancreatic tissue using MBS969811 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human pancreatic tissue using MBS969811 at dilution of 1:100)
Immunofluorescence (IF)
(Immunofluorescent analysis of MCF-7 cells using MBS969811 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunofluorescence (IF)
(Immunofluorescent analysis of MCF-7 cells using MBS969811 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
Uniprot Description
GAPDH: a multifunctional enzyme with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities. A key glycolytic enzyme that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. An important enzyme for energy metabolism, and the production of ATP and pyruvate through anaerobic glycolysis in the cytoplasm. Additionally, it participates in apoptosis, membrane trafficking, iron metabolism, nuclear activities and receptor mediated cell signaling. Its subcellular localization changes reflecting its multiple activities. Is cytosolic, but is also localized in the membrane, the nucleus, polysomes, the ER and the Golgi. Participates in transcription, RNA transport, DNA replication and apoptosis. S-nitrosylation on Cys-152 following apoptotic stimulates its interaction with SIAH2, which in turn moderates its translocation into the nucleus. Mediates cysteine S-nitrosylation of nuclear target proteins including SIRT1, HDAC2 and DNA-PK. Deregulated in lung cancer, renal cancer, breast cancer, gastric cancer, glioma, liver cancer, colorectal cancer, melanoma, prostatic cancer, pancreatic cancer and bladder cancer. Its increased expression and enzymatic activity is associated with cell proliferation and tumorigenesis, Oxidative stress impairs GAPDH catalytic activity and leads to cellular aging and apoptosis. In experimental animal models, injection of GAPDH antagonists induces apoptosis and blocks Hep3B tumor progression, suggesting a therapeutic potential of targeting GAPDH in human hepatocellular carcinoma
Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Oxidoreductase; EC 1.2.1.12
Chromosomal Location of Human Ortholog: 12p13
Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; lipid particle; membrane; microtubule cytoskeleton; nuclear membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; ribonucleoprotein complex; vesicle
Molecular Function: glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity; identical protein binding; microtubule binding; NAD binding; NADP binding; protein binding
Biological Process: carbohydrate metabolic process; gluconeogenesis; glucose metabolic process; microtubule cytoskeleton organization and biogenesis; negative regulation of translation; neuron apoptosis; protein stabilization
Research Articles on GAPDH
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Product Notes
The GAPDH gapdh (Catalog #AAA969811) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Glyceraldehyde-3-phosphate dehydrogenase polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Glyceraldehyde-3-phosphate dehydrogenase can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Recommended dilution: WB:1:500-2000, IHC:1:20-1:200. Researchers should empirically determine the suitability of the GAPDH gapdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glyceraldehyde-3-phosphate dehydrogenase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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