30S ribosomal protein S7 Recombinant Protein | rpsG recombinant protein

Recombinant E Coli 30S ribosomal protein S7

Cat. Num. AAA717356

• Gene Names: rpsG; ECK3328; JW3303
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: 30S ribosomal protein S7; Recombinant E Coli 30S ribosomal protein S7; rpsG recombinant protein

• Host: E Coli
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Liquid containing glycerol
• Sequence Positions: 2-179aa; Full Length
• Sequence: PRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVYSALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMRWIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRWLSLRSFSHQAGASSKQPALGYLN
• Sequence Length: 179

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for rpsG recombinant protein

One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, where it has been shown to contact mRNA. Has been shown to contact tRNA in both the P and E sites; it probably blocks exit of the E site tRNA.

References

The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) The primary structure of ribosomal protein S7 from E. coli strains K and B.Reinbolt J., Tritsch D., Wittmann-Liebold B.Biochimie 61:501-522(1979) DNA sequences from the str operon of Escherichia coli.Post L.E., Nomura M.J. Biol. Chem. 255:4660-4666(1980) Mutant sequences in the rpsL gene of Escherichia coli B/r mechanistic implications for spontaneous and ultraviolet light mutagenesis.Timms A.R., Steingrimsdottir H., Lehmann A.R., Bridges B.A.Mol. Gen. Genet. 232:89-96(1992) Comparison of the complete sequence of the str operon in Salmonella typhimurium and Escherichia coli.Johanson U., Hughes D.Gene 120:93-98(1992) Weigel C.T.O. Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies.Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B.EMBO J. 14:4578-4588(1995) Photochemical cross-linking of initiation factor-3 to Escherichia coli 30 S ribosomal subunits.MacKeen L.A., Kahan L., Wahba A.J., Schwartz I.J. Biol. Chem. 255:10526-10531(1980) Direct cross-links between initiation factors 1, 2, and 3 and ribosomal proteins promoted by 2-iminothiolane.Boileau G., Butler P., Hershey J.W.B., Traut R.R.Biochemistry 22:3162-3170(1983) Assembly of the 30S subunit from Escherichia coli ribosomes occurs via two assembly domains which are initiated by S4 and S7.Nowotny V., Nierhaus K.H.Biochemistry 27:7051-7055(1988) Post-transcriptional regulation of the str operon in Escherichia coli. Ribosomal protein S7 inhibits coupled translation of S7 but not its independent translation.Saito K., Mattheakis L.C., Nomura M.J. Mol. Biol. 235:111-124(1994) The ribosomal neighbourhood of the central fold of tRNA cross-links from position 47 of tRNA located at the A, P or E site.Osswald M., Doering T., Brimacombe R.Nucleic Acids Res. 23:4635-4641(1995) The cross-link from the upstream region of mRNA to ribosomal protein S7 is located in the C-terminal peptide experimental verification of a prediction from modeling studies.Greuer B., Thiede B., Brimacombe R.RNA 5:1521-1525(1999) Tagging ribosomal protein S7 allows rapid identification of mutants defective in assembly and function of 30S subunits.Fredrick K., Dunny G.M., Noller H.F.J. Mol. Biol. 298:379-394(2000) Ribosomal protein S7 from Escherichia coli uses the same determinants to bind 16S ribosomal RNA and its messenger RNA.Robert F., Brakier-Gingras L.Nucleic Acids Res. 29:677-682(2001) Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry.Arnold R.J., Reilly J.P.Anal. Biochem. 269:105-112(1999) Interaction of translation initiation factor 3 with the 30S ribosomal subunit.Dallas A., Noller H.F.Mol. Cell 8:855-864(2001) All-atom homology model of the Escherichia coli 30S ribosomal subunit.Tung C.-S., Joseph S., Sanbonmatsu K.Y.Nat. Struct. Biol. 9:750-755(2002) Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement.Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J.Cell 113:789-801(2003) Structures of the bacterial ribosome at 3.5 A resolution.Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D.Science 310:827-834(2005)

NCBI and Uniprot Product Information

• NCBI GI #: 16131220
• NCBI GeneID: 947846
• NCBI Accession #: NP_417800.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P02359; Q2M706
• Molecular Weight: 23.9 kDa
• NCBI Official Full Name: 30S ribosomal subunit protein S7
• NCBI Official Symbol: rpsG
• NCBI Official Synonym Symbols: ECK3328; JW3303
• NCBI Protein Information: 30S ribosomal subunit protein S7
• UniProt Protein Name: 30S ribosomal protein S7
• Protein Family: 30S ribosomal protein
• UniProt Gene Name: rpsG
• UniProt Entry Name: RS7_ECOLI

NCBI Description

HT_Cmplx17_Mem: [More information is available at EcoGene: EG10906]. The S7 protein is a component of the 30S subunit of the ribosome. [More information is available at EcoCyc: EG10906].

Uniprot Description

One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, where it has been shown to contact mRNA. Has been shown to contact tRNA in both the P and E sites; it probably blocks exit of the E site tRNA.