50S ribosomal protein L6 Recombinant Protein | L6 recombinant protein

Recombinant E Coli 50S ribosomal protein L6

Cat. Num. AAA717252

• Gene Names: rplF; ECK3292; JW3267
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: 50S ribosomal protein L6; Recombinant E Coli 50S ribosomal protein L6; L6 recombinant protein

• Host: E Coli
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Liquid containing glycerol
• Sequence Positions: 2-175aa; Partial
• Sequence: SRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVKHADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNVINLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGKGVRYADEVVRTKEAK
• Sequence Length: 177

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for L6 recombinant protein

This protein binds directly to at least 2 domains of the 23S ribosomal RNA, thus is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center. Gentamicin-resistant mutations in this protein affect translation fidelity.

References

The primary structure of protein L6 from the aminoacyl-tRNA binding site of the Escherichia coli ribosome.Chen R., Arfsten U., Chen-Schmeisser U.Hoppe-Seyler's Z. Physiol. Chem. 358:531-535(1977) The spc ribosomal protein operon of Escherichia coli sequence and cotranscription of the ribosomal protein genes and a protein export gene.Cerretti D.P., Dean D., Davis G.R., Bedwell D.M., Nomura M.Nucleic Acids Res. 11:2599-2616(1983) The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.Link A.J., Robison K., Church G.M.Electrophoresis 18:1259-1313(1997) Alteration of ribosomal protein L6 in gentamicin-resistant strains of Escherichia coli. Effects on fidelity of protein synthesis.Kuehberger R., Piepersberg W., Petzet A., Buckel P., Boeck A.Biochemistry 18:187-193(1979) The use of 2-iminothiolane as an RNA-protein cross-linking agent in Escherichia coli ribosomes, and the localisation on 23S RNA of sites cross-linked to proteins L4, L6, L21, L23, L27 and L29.Wower I., Wower J., Meinke M., Brimacombe R.Nucleic Acids Res. 9:4285-4302(1981) Ribosomal proteins S5 and L6 high-resolution crystal structures and roles in protein synthesis and antibiotic resistance.Davies C., Bussiere D.E., Golden B.L., Porter S.J., Ramakrishnan V., White S.W.J. Mol. Biol. 279:873-888(1998) Escherichia coli proteome analysis using the gene-protein database.VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.Electrophoresis 18:1243-1251(1997) Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry.Arnold R.J., Reilly J.P.Anal. Biochem. 269:105-112(1999) Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y.Mol. Cell. Proteomics 8:215-225(2009) The 3D arrangement of the 23 S and 5 S rRNA in the Escherichia coli 50 S ribosomal subunit based on a cryo-electron microscopic reconstruction at 7.5 A resolution.Mueller F., Sommer I., Baranov P., Matadeen R., Stoldt M., Woehnert J., Goerlach M., van Heel M., Brimacombe R.J. Mol. Biol. 298:35-59(2000) Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement.Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J.Cell 113:789-801(2003) Structures of the bacterial ribosome at 3.5 A resolution.Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D.Science 310:827-834(2005)

NCBI and Uniprot Product Information

• NCBI GI #: 16131184
• NCBI GeneID: 947803
• NCBI Accession #: NP_417764.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P0AG55; P02390; Q2M6X0
• Molecular Weight: 45.5 kDa
• NCBI Official Full Name: 50S ribosomal subunit protein L6
• NCBI Official Symbol: rplF
• NCBI Official Synonym Symbols: ECK3292; JW3267
• NCBI Protein Information: 50S ribosomal subunit protein L6
• UniProt Protein Name: 50S ribosomal protein L6
• Protein Family: 50S ribosomal protein
• UniProt Gene Name: rplF
• UniProt Entry Name: RL6_ECOLI

NCBI Description

The L6 protein is a component of the 50S subunit of the ribosome. [More information is available at EcoCyc: EG10869].

Uniprot Description

This protein binds directly to at least 2 domains of the 23S ribosomal RNA, thus is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.