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PI Reagent

PI Staining Kit

Applications
Immunofluorescence
Synonyms
PI; PI Staining Kit; PI reagent
Ordering
For Research Use Only!
Applicable Applications for PI reagent
Immunofluorescence (IF)
Application Notes
Propidium iodide (PI) has been widely used as a fluorescent stain for DNA in cells; it specificity binds double-stranded nucleic acids in apoptosis, necrosis and fixed cells. PI and DNA complex show light red flourescent color with excitation light 493 nm and emission light 630 nm. It can not throughout the normal living cells membrane, but it enters the apoptosis, necrosis and fixed cells and binds double-stranded DNA.
Preparation and Storage
Store at 4 degree C.
Related Product Information for PI reagent
Background: Propidium iodide (PI) has been widely used as a fluorescent stain for DNA in cells; it specificity binds double-stranded nucleic acids in apoptosis, necrosis and fixed cells. PI and DNA complex show light red flourescent color with excitation light 493 nm and emission light 630 nm. It can not throughout the normal living cells membrane, but it enters the apoptosis, necrosis and fixed cells and binds double-stranded DNA. This product is 1 mg/ml chromogen. Solute it with suitable density for applies. Recommend density is 5 ug/ml.

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Product Notes

The PI (Catalog #AAA826722) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PI can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF). Propidium iodide (PI) has been widely used as a fluorescent stain for DNA in cells; it specificity binds double-stranded nucleic acids in apoptosis, necrosis and fixed cells. PI and DNA complex show light red flourescent color with excitation light 493 nm and emission light 630 nm. It can not throughout the normal living cells membrane, but it enters the apoptosis, necrosis and fixed cells and binds double-stranded DNA. Researchers should empirically determine the suitability of the PI for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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