Rabbit anti-Mouse, Rat SERCA1 Polyclonal Antibody | anti-ATP2A1 antibody

SERCA1 (Sarcoplasmic/endoplasmic Reticulum Calcium ATPase 1, SERCA 1 ATPase, SR Ca(2+) ATPase 1, ATPase Ca++ Transporting Cardiac Muscle Fast Twitch 1, ATPase Ca++ Transporting Fast Twitch 1, ATP2A, ATP2A1, Calcium Pump 1, Calcium Transporting ATPase Sarc

Cat. Num. AAA620697

• Gene Names: ATP2A1; ATP2A; SERCA1
• Reactivity: Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: SERCA1; Polyclonal Antibody; SERCA1 (Sarcoplasmic/endoplasmic Reticulum Calcium ATPase 1; SERCA 1 ATPase; SR Ca(2+) ATPase 1; ATPase Ca++ Transporting Cardiac Muscle Fast Twitch 1; ATPase Ca++ Transporting Fast Twitch 1; ATP2A; ATP2A1; Calcium Pump 1; Calcium Transporting ATPase Sarc; Anti -SERCA1 (Sarcoplasmic/endoplasmic Reticulum Calcium ATPase 1; anti-ATP2A1 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recongnizes endogenous levels of mouse total ATP2A1/SERCA1 protein. Species crossreactivity: rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES pH 7.5, 0.1%, 150mM NaCl, 100ug/ml BSA and 50% glycerol.

• Applicable Applications for anti-ATP2A1 antibody: Western Blot (WB)
• Application Notes: Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
• Immunogen: Synthetic peptide derived from the sequence around Ala988 of mouse ATP2A1/SERCA1 protein (KLH-coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of mouse skeletal muscle extracts using MBS620697.)

Related Product Information for anti-ATP2A1 antibody

Sarcoplasmic and endoplasmic reticulum Ca2+ ATPases (SERCA) are members of a highly conserved family of Ca2+ pumps (1). SERCA pumps transport Ca2+ from the cytosol to the sarcoplasmic and endoplasmic reticulum lumen against a large concentration gradient (1). ATP2A1 (SERCA1) is a fast-twitch, skeletal muscle sarcoplasmic reticulum Ca2+ ATPase (2). Mutations in the correspond-ing gene cause an autosomal recessive muscle disorder known as Brody myopathy, which is characterized by muscle cramping and impaired muscle relaxation associated with exercise (1-3).

Product Categories/Family for anti-ATP2A1 antibody

Antibodies; Abs to Enzymes; ATPase

NCBI and Uniprot Product Information

• NCBI GI #: 10835220
• NCBI GeneID: 487
• NCBI Accession #: NP_004311.1
• NCBI GenBank Nucleotide #: NM_004320.4
• UniProt Accession #: Q7Z675; O14983; Q7Z6E5; Q8N3X5
• Molecular Weight: 109,256 Da
• NCBI Official Full Name: sarcoplasmic/endoplasmic reticulum calcium ATPase 1 isoform a
• NCBI Official Synonym Full Names: ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
• NCBI Official Symbol: ATP2A1
• NCBI Official Synonym Symbols: ATP2A; SERCA1
• NCBI Protein Information: sarcoplasmic/endoplasmic reticulum calcium ATPase 1; calcium pump 1; OTTHUMP00000162561; OTTHUMP00000162562; SR Ca(2+)-ATPase 1; endoplasmic reticulum class 1/2 Ca(2+) ATPase; calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform
• UniProt Protein Name: Putative uncharacterized protein DKFZp779O2152
• Protein Family: Sarcoplasmic/endoplasmic reticulum calcium ATPase
• UniProt Gene Name: DKFZp779O2152
• UniProt Entry Name: Q7Z675_HUMAN

NCBI Description

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]

Uniprot Description

ATP2A1: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. Associated with sarcolipin (SLN) and phospholamban (PLN). Increased contractile activity leads to a decrease in SERCA1 expression, while decreased contractile activity leads to an increase in SERCA1 expression. Skeletal muscle, fast twitch muscle (type II) fibers. Reversibly inhibited by phospholamban (PLN) at low calcium concentrations. Dephosphorylated PLN decreases the apparent affinity of the ATPase for calcium. This inhibition is regulated by the phosphorylation of PLN. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Endoplasmic reticulum; EC 3.6.3.8; Membrane protein, multi-pass; Transporter; Transporter, ion channel; Membrane protein, integral; Hydrolase

Chromosomal Location of Human Ortholog: 16p12.1

Cellular Component: I band; endoplasmic reticulum membrane; sarcoplasmic reticulum membrane; membrane; sarcoplasmic reticulum; perinuclear region of cytoplasm; integral to membrane; ER-Golgi intermediate compartment

Molecular Function: protein binding; protein homodimerization activity; calcium-transporting ATPase activity; calcium ion binding; ATP binding

Biological Process: apoptotic mitochondrial changes; maintenance of mitochondrion localization; positive regulation of fast-twitch skeletal muscle contraction; metabolic process; calcium ion transport; elevation of endoplasmic reticulum calcium ion concentration; reduction of endoplasmic reticulum calcium ion concentration; regulation of striated muscle contraction; blood coagulation; transmembrane transport; negative regulation of striated muscle contraction; elevation of mitochondrial calcium ion concentration

Disease: Brody Myopathy