Immunofluorescent Staining
(Jurkat cells were fixed with cold 70% ethanol and wash twice with cold PBS. Cells were stained with 0.5 mL of PI/RNase Staining Buffer for 30 minutes at room temperature and analyzed by flow cytometry.)
PI / RNase Staining Buffer
PI / RNase Staining Buffer
Applications
Flow Cytometry, Functional Assay
Synonyms
PI / RNase Staining Buffer; PI / RNase Staining Buffer buffer
Form/Format
In PBS (pH7.2), 0.09% NaN3
Applicable Applications for PI / RNase Staining Buffer buffer
Flow Cytometry (FACS)
Usage
This reagent is used to analyze cell cycle by flow cytometry. Use 0.5 ml/test (1×106 cells) and incubate for 30 minutes at room temperature before analysis.
Preparation and Storage
Store undiluted at 4 degree C and protected from prolonged exposure to light. Do not freeze. Do not dilute or boil.
Immunofluorescent Staining
(Jurkat cells were fixed with cold 70% ethanol and wash twice with cold PBS. Cells were stained with 0.5 mL of PI/RNase Staining Buffer for 30 minutes at room temperature and analyzed by flow cytometry.)
Immunofluorescent Staining
(Jurkat cells were fixed with cold 70% ethanol and wash twice with cold PBS. Cells were stained with 0.5 mL of PI/RNase Staining Buffer for 30 minutes at room temperature and analyzed by flow cytometry.)
Related Product Information for PI / RNase Staining Buffer buffer
Propidium iodide (or PI) is an intercalating agent and a fluorescent molecule with a molecular mass of 668.4 Da that can be used to stain cells. When excited with 488 nm wavelength light, it fluoresces red. PI binds to both DNA and RNA, so the latter must be removed by digestion with ribonucleases. The content of DNA as determined by flow cytometry, can reveal useful information about the cell cycle and the proteins involved in the regulation of the cell cycle. This reagent may be used to analyze cell cycle by flow cytometry in addition to use with antibodies for examining the expression of proteins during the cell cycle.
Product Categories/Family for PI / RNase Staining Buffer buffer
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Product Notes
The PI / RNase Staining Buffer (Catalog #AAA355225) is a Buffer and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PI / RNase Staining Buffer can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FACS). Researchers should empirically determine the suitability of the PI / RNase Staining Buffer for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI / RNase Staining Buffer, Buffer" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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