Testing Data #2
(Necrotic, dying, and dead cellsfluoresce green with the newDNA dye CellMetrix GreenLive/Dead Stain.)
CellMetrix Green Live/Dead Dye
CellMetrix Green Live/Dead Dye, 50 uL**
Synonyms
CellMetrix Green Live/Dead; CellMetrix Green Live/Dead Dye; 50 uL**; CellMetrix Green Live/Dead dye
Sample Protocol
Sample Protocol: Typical use: Flow Cytometry 1000 assays - 5 ul of working solution in 495 ul cells (end concentration 50 nM) Imaging 100 assays - 50 ul of working solution in 450 ul cells (end concentration 500 nM) Example Protocol for Flow Cytometry: Spike samples with the Green Live/Dead Stain working solution. Incubate samples approximately 10 min at RT, protected from light. Analyze with a flow cytometer using a blue laser at 488 nm and a 530/30 (FL1) emission filter setting, or similar. Example Protocol for Microscopy: Spike samples with the Green Live/Dead Stain working solution. Incubate samples approximately 10 min at RT, protected from light. When bound to DNA, the peak absorption of CellMetrix Green Live/Dead Stain is 495 nm and the maximum emission is 512 nm. Visualize with a fluorescence microscope using optical filters that best approximate these settings.
Target
Dead Cells, Necrosis
495 nm / >512 nm
When bound to nucleic acids, the maximum absorption of CellMetrix Green Live/Dead Stain is 495 nm and the maximum emission is 512 nm. Cells can be viewed through a fluorescence microscope or analyzed with a flow cytometer.
495 nm / >512 nm
When bound to nucleic acids, the maximum absorption of CellMetrix Green Live/Dead Stain is 495 nm and the maximum emission is 512 nm. Cells can be viewed through a fluorescence microscope or analyzed with a flow cytometer.
Excitation / Emission
Multi-wavelength imaging with UV / vis fluorochromes
Fluorescence enhancement upon dsDNA binding
Compatible with optics of flow, laser scanning cytometers and confocal and lamp-based fluorescence microscopes
Fluorescence enhancement upon dsDNA binding
Compatible with optics of flow, laser scanning cytometers and confocal and lamp-based fluorescence microscopes
Method of Analysis
Flow Cytometer, Fluorescence Microscope
Types of Samples
Cell Culture
Preparation and Storage
Store at -20 degree C
Testing Data #2
(Necrotic, dying, and dead cellsfluoresce green with the newDNA dye CellMetrix GreenLive/Dead Stain.)
Testing Data #2
(Necrotic, dying, and dead cellsfluoresce green with the newDNA dye CellMetrix GreenLive/Dead Stain.)
Related Product Information for CellMetrix Green Live/Dead dye
Description: CellMetrix Green Live/Dead Stain is a new live-cell impermeant, green fluorescence-emitting DNA dye for viability, apoptosis and necrosis studies, and fixed cell nuclear counterstaining. Rapid staining of necrotic or permeabilized cells. Binds to dsDNA/nuclei of DEAD or permeabilized cells. Combination with live cell dyes for live/dead discrimination. Easy to use - no lyse, no wash, no RNase needed. Ideal for use with red SR-FLICA and FLICA 660 caspase assays.
Background: CellMetrix Green Live/Dead Stain is a new live/dead DNA dye for apoptosis and necrosis studies. CellMetrix Green Live/Dead Stain is a membrane impermeant DNA dye for the rapid and convenient staining of the nuclear DNA of dead or fixed cells. This new green DNA dye is an ideal live/dead stain to assess necrosis in cytotoxicity studies, as well as a reliable DNA dye in fixed cell samples with immunofluorescence, immunohistochemistry, and high content screening applications. This green Live/Dead Stain is a recently developed vital dye that exhibits intact cell membrane exclusion properties analogous to the popular red fluorescing vital dyes, Propidium Iodide (PI), 7-aminoactinomycinD (7-AAD), and DRAQ7. Like the red fluorescing DNA dyes, CellMetrix Green Live/Dead Stain is excluded from intact, healthy cells due to its polar nature. In the presence of cells exhibiting compromised membrane integrity, CellMetrix Green Live/Dead Stain penetrates the cell and nuclear membrane barriers and intercalates tightly to DNA in a manner analogous to PI and 7-AAD. When bound to DNA, it acquires a greatly enhanced fluorescence potential (>2000X) in the green emission range. These important vital dye properties enable CellMetrix Green Live/Dead Stain to be used in flow cytometry-based protocols and microscopy analysis techniques to assess the percentage of late apoptotic, necrotic, and membrane-compromised cells within a sample cell population. CellMetrix Green Live/Dead Stain is supplied as a concentrated stock solution. Each 50 ul vial is sufficient for 1000 flow cytometry tests or 100 microtiter plate wells per our sample protocol. Optimal Ex/ Em: 495 nm/ 512nm Identification of Dead Cells / Membrane-Compromised Cells: Live Cell Membrane-Impermeant CellMetrix Green Live/Dead Stain only stains nuclei in dead and permeabilized cells. CellMetrix Green Live/Dead Stain does not enter intact, live cells. Cells may be fixed or permeabilized for use of CellMetrix Green Live/Dead Stain as a nuclear counterstain. Green emission The green fluorescence emission (512 nm optimal) of CellMetrix Green Live/Dead Stain permits new combinations of dyes to be used in the understanding of cell death and apoptosis. CellMetrix Green Live/Dead Stain is ideal for use with red SR-FLICA assays, far-red FLICA 660 caspase assays, and other visible range fluor-tagged ligands. Non-toxic in long-term culture With negligible toxicity, CellMetrix Green Live/Dead Stain can be used in long-term viability assays to assess cytotoxicity and apoptosis. Nuclear Counterstaining with Fixed Cells & Tissue Sections: For microscopy applications, CellMetrix Green Live/Dead Stain will function as a DNA counterstain for fixed cell and tissue samples in IF, IHC, high content screening, and cell-based assays. Visible Range Excitation: Unlike blue fluorescence DNA dyes, CellMetrix Green Live/Dead Stain is excited with a blue laser in a flow cytometer and blue excitation filters in imaging instruments. Make compound screens more effective: Visible range excitation avoids a "white-out" effect from coincidental UV fluorescence of compounds when using DAPI or Hoechst DNA dyes. Spectrally compatible with UV excited fluors Compatible with PE-tagged probes, SR-FLICA, or FLICA 660 caspase assays.
Background: CellMetrix Green Live/Dead Stain is a new live/dead DNA dye for apoptosis and necrosis studies. CellMetrix Green Live/Dead Stain is a membrane impermeant DNA dye for the rapid and convenient staining of the nuclear DNA of dead or fixed cells. This new green DNA dye is an ideal live/dead stain to assess necrosis in cytotoxicity studies, as well as a reliable DNA dye in fixed cell samples with immunofluorescence, immunohistochemistry, and high content screening applications. This green Live/Dead Stain is a recently developed vital dye that exhibits intact cell membrane exclusion properties analogous to the popular red fluorescing vital dyes, Propidium Iodide (PI), 7-aminoactinomycinD (7-AAD), and DRAQ7. Like the red fluorescing DNA dyes, CellMetrix Green Live/Dead Stain is excluded from intact, healthy cells due to its polar nature. In the presence of cells exhibiting compromised membrane integrity, CellMetrix Green Live/Dead Stain penetrates the cell and nuclear membrane barriers and intercalates tightly to DNA in a manner analogous to PI and 7-AAD. When bound to DNA, it acquires a greatly enhanced fluorescence potential (>2000X) in the green emission range. These important vital dye properties enable CellMetrix Green Live/Dead Stain to be used in flow cytometry-based protocols and microscopy analysis techniques to assess the percentage of late apoptotic, necrotic, and membrane-compromised cells within a sample cell population. CellMetrix Green Live/Dead Stain is supplied as a concentrated stock solution. Each 50 ul vial is sufficient for 1000 flow cytometry tests or 100 microtiter plate wells per our sample protocol. Optimal Ex/ Em: 495 nm/ 512nm Identification of Dead Cells / Membrane-Compromised Cells: Live Cell Membrane-Impermeant CellMetrix Green Live/Dead Stain only stains nuclei in dead and permeabilized cells. CellMetrix Green Live/Dead Stain does not enter intact, live cells. Cells may be fixed or permeabilized for use of CellMetrix Green Live/Dead Stain as a nuclear counterstain. Green emission The green fluorescence emission (512 nm optimal) of CellMetrix Green Live/Dead Stain permits new combinations of dyes to be used in the understanding of cell death and apoptosis. CellMetrix Green Live/Dead Stain is ideal for use with red SR-FLICA assays, far-red FLICA 660 caspase assays, and other visible range fluor-tagged ligands. Non-toxic in long-term culture With negligible toxicity, CellMetrix Green Live/Dead Stain can be used in long-term viability assays to assess cytotoxicity and apoptosis. Nuclear Counterstaining with Fixed Cells & Tissue Sections: For microscopy applications, CellMetrix Green Live/Dead Stain will function as a DNA counterstain for fixed cell and tissue samples in IF, IHC, high content screening, and cell-based assays. Visible Range Excitation: Unlike blue fluorescence DNA dyes, CellMetrix Green Live/Dead Stain is excited with a blue laser in a flow cytometer and blue excitation filters in imaging instruments. Make compound screens more effective: Visible range excitation avoids a "white-out" effect from coincidental UV fluorescence of compounds when using DAPI or Hoechst DNA dyes. Spectrally compatible with UV excited fluors Compatible with PE-tagged probes, SR-FLICA, or FLICA 660 caspase assays.
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Product Notes
The CellMetrix Green Live/Dead (Catalog #AAA258043) is a Dye and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CellMetrix Green Live/Dead, Dye" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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