Testing Data
(NIH-3T3 cells were grown in 96 well plates, fixed with paraformaldehyde and permeabilized. Then incubate with DAPI solution for 5 minutes. )
DAPI Staining Solution IHC Related Reagent
DAPI Staining Solution
Synonyms
DAPI Staining Solution; DAPI Staining Solution ihc related reagent
Protocol
1. As for the fixed cell and tissue, wash appropriately to remove the fixing agent. If necessary, immunofluorescent staining can be performed first, then perform the DAPI staining. If there is no other staining, perform DAPI staining directly. 2. As for the adherent cell or tissue slice, add small volume of DAPI staining solution directly (overlying the sample is enough, about 50-100ul). But for the suspending cell, add at least 3 times volume of DAPI staining solution comparing with the sample and mix thoroughly. 3. Incubate for 5-10 minutes at RT(room temperature) 4. Pipette the DAPI out, and wash with TBST or PBS or physiological saline for 2-3 times, 3-5 minutes each to remove the unbinding DAPI. 5. Observe the cell under the fluorescence microscope with optical filter owning 360nm excitation wavelength and 460nm emission wavelength.
Product Note
1. Since all fluorescent dyes have the problem of cancellation, we suggest finish detecting within the day of staining. 2. Can use the Anti-fade solution (AR1109) to retard the cancellation of fluorescent dyes. 3. Please pay attention to the irritation of DAPI. 4. Please operate with gloves.
Preparation and Storage
Store at -20 degree C in dark for one year.
Testing Data
(NIH-3T3 cells were grown in 96 well plates, fixed with paraformaldehyde and permeabilized. Then incubate with DAPI solution for 5 minutes. )
Testing Data
(NIH-3T3 cells were grown in 96 well plates, fixed with paraformaldehyde and permeabilized. Then incubate with DAPI solution for 5 minutes. )
Related Product Information for DAPI Staining Solution ihc related reagent
DAPI (4',6-diamidino-2-phenylindole) is a kind of fluorescent dye which can bind DNA strands robustly, and the fluorescence can be detected by fluorescence microscope. DAPI can dye live cells and fixed cells as it can transmit whole membrane. The molecular formula is C16H15N5;2HCl with 350.25 molecular weight, CAS Number 28718-90-3. DAPI could transmit cell membrane and bind the double-strand DNA in the nucleus, and produce 20 times stronger fluorescence than itself. The sensitivity for double stranded DNA staining is many times larger comparing with EB. Blue fluorescent cell would be seen under the microscope. The efficiency detected by fluorescence microscope is very high(almost 100%),and there is no side effect for the live cells. DAPI staining is usually used in cell death detection. After staining with DAPI, detect with fluorescence microscope or flow cytometry. DAPI is also usually used in nucleus staining and double-strand DNA staining in some special situation. After heat treatment, using DAPI stain the cell for 3min,then morphological changes of the nucleus can be detected the under the fluorescence microscope. The largest excitation wavelength for DAPI is 340nm,and the largest emission wavelength is 488nm;When DAPI binds with double-strand DNA, the largest excitation wavelength is 360nm,while the the largest emission wavelength becomes 460nm. This DAPI Staining Solution can be used for fixed cells or nucleus of tissue staining directly.
Product Categories/Family for DAPI Staining Solution ihc related reagent
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Product Notes
The DAPI Staining Solution (Catalog #AAA176580) is an IHC Related Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "DAPI Staining Solution, IHC Related Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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