Human Galectin-1 ELISA Kit | LGALS1 elisa kit

Human Galectin-1 PicoKine ELISA Kit

Cat. Num. AAA178519

• Gene Names: LGALS1; GBP; GAL1
• Reactivity: Human
• Synonyms: Galectin-1; Human Galectin-1 PicoKine ELISA Kit; Galaptin; Galectin 1; Galectin1; Galectin1(Gal1); Galectin-1(Gal-1); HBL; HLBP14; HPL; Lactose binding lectin 1; Lactose-binding lectin 1; LEG1; LGALS1; S Lac lectin 1; S-Lac lectin 1; P09382; lectin; galactoside-binding; soluble; 1; LGALS1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human Galectin-1
• Sequence Length: 135

• Samples: Cell culture supernates, serum and plasma(heparin, EDTA).
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10000pg/ml
• Sensitivity: <15pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).

Typical Testing Data/Standard Curve (for reference only)

(Human Galectin-1 PicoKine ELISA Kit standard curve )

Related Product Information for LGALS1 elisa kit

Principle of the Assay: Human Galectin-1 ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for Galectin-1 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: A2-D135) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Galectin-1 amount of sample captured in plate.

Background/Introduction: Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Its expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ Tcells. Additionally, Galectin-1 is thought to play a role in creating immune tolerance in pregnancy.

NCBI and Uniprot Product Information

• NCBI GI #: 4504981
• NCBI GeneID: 3956
• NCBI Accession #: NP_002296.1
• NCBI GenBank Nucleotide #: NM_002305.3
• UniProt Accession #: P09382; Q9UDK5; B2R5E8
• Molecular Weight: 14,716 Da
• NCBI Official Full Name: galectin-1
• NCBI Official Synonym Full Names: galectin 1
• NCBI Official Symbol: LGALS1
• NCBI Official Synonym Symbols: GBP; GAL1
• NCBI Protein Information: galectin-1
• UniProt Protein Name: Galectin-1
• Protein Family: Galectin
• UniProt Gene Name: LGALS1
• UniProt Synonym Gene Names: Gal-1; HLBP14
• UniProt Entry Name: LEG1_HUMAN

NCBI Description

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

galectin-1: May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Homodimer. Binds LGALS3BP. Interacts with CD2, CD3, CD4, CD7, CD43 and CD45. Interacts with laminin (via poly-N- acetyllactosamine). Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion. Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.

Protein type: Extracellular matrix; Secreted; Cell adhesion

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cytoplasm; extracellular matrix; extracellular space; intracellular

Molecular Function: protein binding; signal transducer activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of virion penetration into host cell; regulation of apoptosis