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Typical Testing Data/Standard Curve (for reference only)

Mouse GFRA3 ELISA Kit | GFRA3 elisa kit

Mouse GFRA3 PicoKine ELISA Kit

Gene Names
Gfra3; Y15110; GFRalpha3
Reactivity
Mouse
Synonyms
GFRA3; Mouse GFRA3 PicoKine ELISA Kit; Mouse GFRA3; glial cell line derived neurotrophic factor family receptor alpha 3; GDNF family receptor alpha-3; GDNF receptor alpha-3; GDNFR-alpha-3; GFR-alpha-3; Gfra3; GFRA3 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Gfra3
Sequence Length
1867
Samples
Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GFRA3 elisa kit
Principle of the Assay: The Picokine™ Mouse Gfra3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Gfra3 with a 96-well strip plate that is pre-coated with antibody specific for Gfra3. The detection antibody is a biotinylated antibody specific for Gfra3. The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Mouse Gfra3 with immunogen: Expression system for standard: NSO, Immunogen sequence: N29-N371. The kit is analytically validated with ready to use reagents. To measure Mouse Gfra3, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Gfra3 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Gfra3 in the sample.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Mus musculus mRNA for GFRalpha-3, complete cds
NCBI Official Synonym Full Names
glial cell line derived neurotrophic factor family receptor alpha 3
NCBI Official Symbol
Gfra3
NCBI Official Synonym Symbols
Y15110; GFRalpha3
NCBI Protein Information
GDNF family receptor alpha-3
UniProt Protein Name
GDNF family receptor alpha-3
Protein Family
UniProt Gene Name
Gfra3
UniProt Synonym Gene Names
GDNF receptor alpha-3; GDNFR-alpha-3; GFR-alpha-3
UniProt Entry Name
GFRA3_MOUSE

NCBI Description

This gene encodes a cell surface glycoprotein and member of the glial cell line-derived neurotrophic receptor (GDNFR) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein mediates binding of the ligand artemin to the ret receptor tyrosine kinase, and this interaction may regulate thermal pain and axon regeneration. Homozygous knockout mice for this gene exhibit impaired proliferation of cultured neuroblasts and impaired development of the superior cervical ganglion. [provided by RefSeq, Aug 2015]

Uniprot Description

GFRA3: Receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase. Belongs to the GDNFR family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Cellular Component: membrane; intracellular membrane-bound organelle; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; coreceptor activity; receptor activity; axon guidance receptor activity

Biological Process: axon guidance; sympathetic nervous system development; neuron migration; neuron development; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway

Research Articles on GFRA3

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Product Notes

The Mouse GFRA3 gfra3 (Catalog #AAA1752681) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1752681 ELISA Kit recognizes Mouse GFRA3. It is sometimes possible for the material contained within the vial of "GFRA3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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