Rat BMP-2 ELISA Kit | BMP-2 elisa kit

Rat BMP-2 PicoKine Fast ELISA Kit

Cat. Num. AAA1752709

• Reactivity: Rat
• Synonyms: BMP-2; Rat BMP-2 PicoKine Fast ELISA Kit; Rat BMP-2; Bone morphogenetic protein 2; Bone morphogenetic protein 2A; BMP-2A; Bmp2; Bmp-2; BMP-2 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant Rat Bmp2
• Sequence Length: 393

• Samples: Bone tissue, cell culture supernates and serum
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <2pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP-2 elisa kit

Principle of the Assay: The Picokine™ Rat Bmp2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Bmp2 with a 96-well strip plate that is pre-coated with antibody specific for Bmp2. The detection antibody is a biotinylated antibody specific for Bmp2. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Rat Bmp2 with immunogen: Expression system for standard: CHO; Immunogen sequence: Q283-R396. The kit is analytically validated with ready to use reagents. To measure Rat Bmp2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Rat Bmp2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Bmp2 in the sample.!!Background/Introduction: Bone morphogenetic protein-2(BMP-2, BMP 2A) belongs to the transforming growth factor beta(TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice. BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase(MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis. Bone morphogenic proteins(BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion. The standard product used in this kit is recombinant rat BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

NCBI and Uniprot Product Information

• NCBI GI #: 1345612
• NCBI GeneID: 29373
• UniProt Accession #: P49001
• NCBI Official Full Name: Bone morphogenetic protein 2
• NCBI Official Synonym Full Names: bone morphogenetic protein 2
• NCBI Official Symbol: Bmp2
• NCBI Protein Information: bone morphogenetic protein 2
• UniProt Protein Name: Bone morphogenetic protein 2
• Protein Family: BMP-2-inducible protein kinase
• UniProt Gene Name: Bmp2
• UniProt Synonym Gene Names: Bmp-2; BMP-2; BMP-2A
• UniProt Entry Name: BMP2_RAT

NCBI Description

involved in cellular signaling during limb development; induces bone formation [RGD, Feb 2006]

Uniprot Description

BMP2: Induces cartilage and bone formation. Belongs to the TGF-beta family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Cellular Component: extracellular space; protein complex; cell surface; cytoplasm; extracellular region

Molecular Function: protein domain specific binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; phosphatase activator activity; cytokine activity; SMAD binding; transforming growth factor beta receptor binding; receptor binding; retinol dehydrogenase activity

Biological Process: activation of MAPK activity; positive regulation of apoptosis; heart development; positive regulation of transcription, DNA-dependent; adrenocorticotropin hormone secreting cell differentiation; telencephalon regionalization; protein amino acid phosphorylation; cardiac muscle cell differentiation; regulation of apoptosis; BMP signaling pathway; ovulation cycle; chondrocyte differentiation; inner ear development; regulation of odontogenesis of dentine-containing teeth; positive regulation of astrocyte differentiation; pericardium development; positive regulation of neurogenesis; cell fate commitment; organ morphogenesis; mesenchymal cell differentiation; response to mechanical stimulus; regulation of MAPKKK cascade; positive regulation of osteoblast proliferation; positive regulation of fat cell differentiation; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of cell differentiation; negative regulation of calcium-independent cell-cell adhesion; positive regulation of odontogenesis; proteoglycan metabolic process; embryonic heart tube anterior/posterior pattern formation; negative regulation of insulin-like growth factor receptor signaling pathway; thyroid stimulating hormone secreting cell differentiation; negative regulation of transcription from RNA polymerase II promoter; bone mineralization; negative regulation of cell cycle; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; positive regulation of MAPKKK cascade; negative regulation of aldosterone biosynthetic process; cardiac muscle morphogensis; inflammatory response; positive regulation of Wnt receptor signaling pathway; cardiac cell differentiation; Notch signaling pathway; ossification; response to retinoic acid; protein destabilization; in utero embryonic development; positive regulation of bone mineralization; positive regulation of ossification; odontogenesis of dentine-containing teeth; osteoblast differentiation; positive regulation of osteoblast differentiation; telencephalon development; ureteric bud branching; cartilage development; response to hypoxia; epithelial to mesenchymal transition; positive regulation of protein amino acid phosphorylation; positive regulation of neuron differentiation; growth; positive regulation of cell migration