BMP-2 elisa kit

Monkey primate BMP-2 PicoKine ELISA Kit

Cat. Num. AAA1751025

• Gene Names: BMP2; BDA2; BMP2A
• Reactivity: Monkey; There is no detectable cross-reactivity with other relevant proteins.
• Applications: ELISA
• Synonyms: BMP-2; Monkey primate BMP-2 PicoKine ELISA Kit; Bone morphogenetic protein 2; BMP2; BMP-2 elisa kit

• Reactivity: Monkey; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant 215 Monkey primate BMP-2
• Sequence Length: 396

• Applicable Applications for BMP-2 elisa kit: ELISA (EIA)
• Assay Type: Quantitative Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <2pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration weretested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration weretested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP-2 elisa kit

Principle of the Assay: 215 Monkey primate BMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Monkey primate BMP-2 has been precoated onto 96-well plates. Standards(Expression system for standard: CHO; Immunogen sequence: Q283-R396) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Monkey primate BMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the 215 Monkey primate BMP-2 amount of sample captured in plate.

Background: Bone morphogenetic protein-2(BMP-2, BMP 2A) belongs to the transforming growth factor beta(TGF- beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase(MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins(BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant monkey primate BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

Product Categories/Family for BMP-2 elisa kit

Cardiovascular; Angiogenesis; Growth Factors; TGF; Signal Transduction; Growth Factors/Hormones; Cytoskeleton/ECM; Extracellular Matrix; Structures; Bone; Stem Cells; Signaling Pathways; TGF Beta; Secreted; Neural Stem Cells; Extracellular; Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 4557369
• NCBI GeneID: 650
• NCBI Accession #: NP_001191.1
• NCBI GenBank Nucleotide #: NM_001200.3
• UniProt Accession #: C8C060; P12643
• Molecular Weight: 44702 MW
• NCBI Official Full Name: bone morphogenetic protein 2 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 2
• NCBI Official Symbol: BMP2
• NCBI Official Synonym Symbols: BDA2; BMP2A
• NCBI Protein Information: bone morphogenetic protein 2
• UniProt Protein Name: Bone morphogenetic protein 2
• Protein Family: BMP-2-inducible protein kinase
• UniProt Gene Name: BMP2

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. Duplication of a regulatory region downstream of this gene causes a form of brachydactyly characterized by a malformed index finger and second toe in human patients. [provided by RefSeq, Jul 2016]