Human Haptoglobin ELISA Kit | HP elisa kit

Human Haptoglobin PicoKine ELISA Kit

Cat. Num. AAA1751607

• Gene Names: HP; BP; HPA1S; HP2ALPHA2
• Reactivity: Human
• Synonyms: Haptoglobin; Human Haptoglobin PicoKine ELISA Kit; Human Haptoglobin; HP elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human GPC5
• Sequence Length: 406

• Samples: Cell Culture Supernates, Serum And Plasma (Heparin, Edta, Citrate)
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HP elisa kit

Principle of the Assay: The Picokine Human GPC5 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human GPC5 with a 96-well strip plate that is pre-coated with antibody specific for GPC5. The detection antibody is a biotinylated antibody specific for GPC5. The capture antibody is A monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Human GPC5 with immunogen: Expression system for standard: NSO, Immunogen sequence: E25-T554. The kit is analytically validated with ready to use reagents. To measure Human GPC5, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human GPC5 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human GPC5 in the sample!!Background/Introduction: Glypican-5 is a protein that in humans is encoded by the GPC5 gene. This gene is mapped to 13q31.3. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypicanrelated integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

NCBI and Uniprot Product Information

• NCBI GI #: 386783
• NCBI GeneID: 3240
• NCBI Official Full Name: haptoglobin
• NCBI Official Synonym Full Names: haptoglobin
• NCBI Official Symbol: HP
• NCBI Official Synonym Symbols: BP; HPA1S; HP2ALPHA2
• NCBI Protein Information: haptoglobin
• Protein Family: Haptoglobin

NCBI Description

This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. The protein encoded also exhibits antimicrobial activity against bacteria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]