Human CDNF ELISA Kit | CDNF elisa kit

Human CDNF PicoKine ELISA Kit

Cat. Num. AAA1751575

• Gene Names: CDNF; ARMETL1
• Reactivity: Human
• Synonyms: CDNF; Human CDNF PicoKine ELISA Kit; Human CDNF; CDNF elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human CDCP1
• Sequence Length: 187

• Samples: Cell Culture Supernates, Serum And Plasma (Heparin, Edta, Citrate)
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <2pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CDNF elisa kit

Principle of the Assay: The Picokine Human CDCP1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CDCP1 with a 96-well strip plate that is pre-coated with antibody specific for CDCP1. The detection antibody is a biotinylated antibody specific for CDCP1. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human CDCP1 with immunogen: Expression system for standard: NSO, Immunogen sequence: F30-T667. The kit is analytically validated with ready to use reagents. To measure Human CDCP1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human CDCP1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CDCP1 in the sample!!Background/Introduction: CUB domain-containing protein 1 (CDCP1) is a protein that in humans is encoded by the CDCP1 gene. It has also been designated as CD318 (cluster of differentiation 318) and Trask (Transmembrane and associated with src kinases). CDCP1/Trask is a 140 kD transmembrane glycoprotein with a large extracellular domain (ECD) containing two CUB domains, and a smaller intracellular domain (ICD) containing five tyrosines. The tyrosine phosphorylation of Trask is tightly regulated and reciprocally linked with the state of cell adhesion. The tyrosine phosphorylation of CDCP1 in cultured cells occurs when cells are induced to detach by trypsin or EDTA, or seen spontaneously during mitotic detachment. The overexpression of CDCP1 leads to the loss of cell adhesion and a detached phenotype. CDCP1 is widely expressed in human epithelial tissues, but its phosphorylation is only seen in mitotically detached or shedding cells, consistent with its role in the negative regulation of cell adhesion.

NCBI and Uniprot Product Information

• NCBI GI #: 82880674
• NCBI GeneID: 441549
• NCBI Accession #: NP_001025125.2
• NCBI GenBank Nucleotide #: NM_001029954.3
• UniProt Accession #: Q49AH0
• NCBI Official Full Name: cerebral dopamine neurotrophic factor
• NCBI Official Synonym Full Names: cerebral dopamine neurotrophic factor
• NCBI Official Symbol: CDNF
• NCBI Official Synonym Symbols: ARMETL1
• NCBI Protein Information: cerebral dopamine neurotrophic factor
• Protein Family: Cerebral dopamine neurotrophic factor