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Western Blot (WB) (Western blot analysis of Tle6 on different lysates using anti-Tle6 antibody at 1/500 dilution. Positive control�� Lane1: Mouse ovary tissue Lane2: HepG2)

Rabbit anti-Human, Mouse Tle6 Polyclonal Antibody | anti-Tle6 antibody

Tle6 Antibody

Gene Names
TLE6; GRG6
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide affinity purified.
Synonyms
Tle6; Polyclonal Antibody; Tle6 Antibody; anti-Tle6 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Peptide affinity purified.
Form/Format
1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-Tle6 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Immunogen
Synthetic peptide within mouse Tle6 aa 150-200.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Tle6 on different lysates using anti-Tle6 antibody at 1/500 dilution. Positive control�� Lane1: Mouse ovary tissue Lane2: HepG2)

Western Blot (WB) (Western blot analysis of Tle6 on different lysates using anti-Tle6 antibody at 1/500 dilution. Positive control�� Lane1: Mouse ovary tissue Lane2: HepG2)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Tle6 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Tle6 antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining Tle6 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining Tle6 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining Tle6 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining Tle6 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining Tle6 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining Tle6 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Daudi cells with Tle6 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of Daudi cells with Tle6 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Related Product Information for anti-Tle6 antibody
The Notch signaling pathway controls various cellular interactions that are important for the specification of a variety of fates in both vertebrates and invertebrates. Key players in the Notch pathway are the TLE genes (for transducin-like enhancer of split, also designated ESG for enhancer of split groucho), which are human homologs of the Drosophila groucho gene. TLE6 (transducin-like enhancer of split 6), also known as GRG6, is a 449 amino acid cytoplasmic protein belonging to the WD repeat groucho/TLE family. As a member of the subcortical maternal complex (SCMC), TLE6 is essential for zygotes to progress beyond the first embryonic cell divisions. TLE6 contains seven WD repeats, a motif known to mediate protein-protein interactions. The WD40 repeat family of proteins is suggested to be involved in signal transduction, RNA processing, gene regulation, vesicular trafficking, cytoskeletal assembly and may play a role in the control of cytotypic differentiation.
Product Categories/Family for anti-Tle6 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
63,473 Da
NCBI Official Full Name
transducin-like enhancer protein 6 isoform 1
NCBI Official Synonym Full Names
transducin-like enhancer of split 6 (E(sp1) homolog, Drosophila)
NCBI Official Symbol
TLE6
NCBI Official Synonym Symbols
GRG6
NCBI Protein Information
transducin-like enhancer protein 6
UniProt Protein Name
Transducin-like enhancer protein 6
UniProt Gene Name
TLE6
UniProt Entry Name
TLE6_HUMAN

Uniprot Description

TLE6: As a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions. Belongs to the WD repeat Groucho/TLE family.

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: protein complex; cell cortex; nucleus

Biological Process: regulation of transcription, DNA-dependent; positive regulation of neuron differentiation

Research Articles on Tle6

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Product Notes

The Tle6 tle6 (Catalog #AAA9435387) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Tle6 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Tle6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:1000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the Tle6 tle6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tle6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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