Rabbit anti-Mouse TRIAD3A (TLR-Ubiquitinating Enzyme) Polyclonal Antibody | anti-TRIAD3A antibody

TRIAD3A (TLR-Ubiquitinating Enzyme)

Cat. Num. AAA395531

• Gene Names: RNF216; ZIN; U7I1; TRIAD3; UBCE7IP1
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Antigen Immunoaffiinity Purification
• Synonyms: TRIAD3A (TLR-Ubiquitinating Enzyme); Polyclonal Antibody; EC 6.3.2.; Ubiquitin-conjugating enzyme 7-interacting protein 1; Zinc finger protein inhibiting NF-kappa-B; Triad domain-containing protein 3; anti-TRIAD3A antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Immunoaffiinity Purification
• Form/Format: Provided as solution in phosphate buffered saline with 0.02% sodium azide
• Sequence Length: 923

• Applicable Applications for anti-TRIAD3A antibody: Western Blot (WB)
• Immunogen: Rabbit polyclonal TRIAD3A antibody was raised against a peptide corresponding to 15 amino acids near the amino-terminus of mouse TRIAD3A.
• Positive Control: Located in the cytoplasm, TRIAD3A is ubiquitous, with the highest levels of expression in testis and peripheral blood leukocytes.6
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Western Blot/Blotting

(Western blot analysis of TRIAD3A in mouse heart cell lysates with TRIAD3A antibody at (A) 0.5, (B) 1, and (C) 2 g/ml)

Related Product Information for anti-TRIAD3A antibody

Activation of NF-?B as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses (reviewed in 1). Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 (2). Several regulatory mechanisms exist to control TLR signal transduction, including the inhibition of TLR expression and signaling by molecules such as ST2 and SIGIRR (3,4). Another mechanism is by the ubi-quitinization of selected TLRs by TRIAD3A, an E3 ubiquitin-protein ligase (5). TRIAD3A is a RING finger protein that can bind to TLR4 and TLR9, and to a lesser extent TLR3 and TLR5, catalyzing the ubiquitization of these molecules. Overexpression of TRIAD3A promoted the nearly complete degradation of TLR4 and TLR9; this reduction was reflected in the decreased signal-specific activation by ligands specific for these TLRs. Conversely, depletion of TRIAD3A resulted in enhanced TLR activation (5).

Product Categories/Family for anti-TRIAD3A antibody

Polyclonal Antibody; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 46358403
• NCBI GeneID: 54476
• NCBI Accession #: NP_996994.1
• NCBI GenBank Nucleotide #: NM_207111.3
• UniProt Accession #: Q9NWF9; Q6Y691; Q75ML7; Q7Z2H7; Q7Z7C1; Q8NHW7; Q9NYT1
• Molecular Weight: 99 kD
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF216 isoform a
• NCBI Official Synonym Full Names: ring finger protein 216
• NCBI Official Symbol: RNF216
• NCBI Official Synonym Symbols: ZIN; U7I1; TRIAD3; UBCE7IP1
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF216; triad domain-containing protein 3; zinc finger protein inhibiting NF-kappa-B; ubiquitin-conjugating enzyme 7-interacting protein 1
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF216
• UniProt Gene Name: RNF216
• UniProt Synonym Gene Names: TRIAD3; UBCE7IP1; ZIN
• UniProt Entry Name: RN216_HUMAN

NCBI Description

This gene encodes a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates. Several alternatively spliced transcript variants have been described for this locus but the full-length natures of only some are known. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Isoform 1 acts as an E3 ubiquitin ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Promotes degradation of TRAF3, TLR4 and TLR9. Contributes to the regulation of antiviral responses. Down-regulates activation of NF-kappa-B, IRF3 activation and IFNB production. Isoform 3/ZIN inhibits TNF and IL-1 mediated activation of NF-kappa-B. Promotes TNF and RIP mediated apoptosis. Ref.2 Ref.9

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Interacts with UBE2L3 and to some extent with UBE2L6. Interacts with TRAF3, TLR3, TLR4, TLR5 and TLR9. Isoform 3/ZIN binds RIPK1 and HIV VIF. Ref.1 Ref.2 Ref.8 Ref.9

Subcellular location: Cytoplasm.

Tissue specificity: Ubiquitous, with the highest levels of expression in testis and peripheral blood leukocytes.

Domain: The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme

By similarity.

Post-translational modification: Auto-ubiquitinated.

Sequence similarities: Contains 1 IBR-type zinc finger.Contains 2 RING-type zinc fingers.

Sequence caution: The sequence BAA91422.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.