Western Blot/Blotting
(Western blot analysis of IRF7 in 293 whole cell lysate with IRF7 antibody at (A) 0.5 and (B) 1 ug/ml.)
Rabbit anti-Human Interferon regulatory factor 7/IRF7 Polyclonal Antibody | anti-IRF7 antibody
Interferon regulatory factor 7 (IN)/IRF7
Western Blot/Blotting
(Western blot analysis of IRF7 in 293 whole cell lysate with IRF7 antibody at (A) 0.5 and (B) 1 ug/ml.)
Western Blot/Blotting
(Western blot analysis of IRF7 in 293 whole cell lysate with IRF7 antibody at (A) 0.5 and (B) 1 ug/ml.)
NCBI and Uniprot Product Information
NCBI Description
IRF7 encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway. Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase. Exists in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Can also play a role in regulating adaptive immune responses by inducing PSMB9/LMP2 expression, either directly or through induction of IRF1. Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages. Ref.6 Ref.8 Ref.16 Ref.21
Enzyme regulation: In the absence of viral infection, maintained as a monomer in an autoinhibited state and phosphorylation disrupts this autoinhibition leading to the liberation of the DNA-binding and dimerization activities and its nuclear localization where it can activate type I IFN and ISG genes.
Subunit structure: Monomer. Homodimer; phosphorylation-induced. Heterodimer with IRF3. Interacts with TICAM1 and TICAM2. Interacts with rotavirus A NSP1; this interaction leads to the proteasome-dependent degradation of IRF7. Interacts with Epstein-Barr virus LF2. Interacts with MYD88 AND TRAF6. Ref.6 Ref.10 Ref.12 Ref.15 Ref.16 Ref.22 Ref.23
Subcellular location: Nucleus. Cytoplasm. Note: The phosphorylated and active form accumulates selectively in the nucleus. Ref.6 Ref.15 Ref.16
Tissue specificity: Expressed predominantly in spleen, thymus and peripheral blood leukocytes.
Induction: By type I interferon (IFN) and viruses.
Post-translational modification: Acetylation inhibits its DNA-binding ability and activity.In response to a viral infection, phosphorylated on Ser-477 and Ser-479 by TBK1 and IKBKE1. Phosphorylation, and subsequent activation is inhibited by vaccinia virus protein E3. In TLR7- and TLR9-mediated signaling pathway, phosphorylated by IRAK1. Ref.6 Ref.7 Ref.8 Ref.14 Ref.17TRAF6-mediated ubiquitination is required for IRF7 activation
By similarity.Sumoylated by TRIM28, which inhibits its transactivation activity. Ref.26
Sequence similarities: Belongs to the IRF family.Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
Research Articles on IRF7
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Product Notes
The IRF7 irf7 (Catalog #AAA395467) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Interferon regulatory factor 7 (IN)/IRF7 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Interferon regulatory factor 7/IRF7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the IRF7 irf7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interferon regulatory factor 7/IRF7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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