Testing Data
(BLM antibody detects BLM protein by western blot analysis.A. 30 mug Jurkat whole cell lysate/extract B. 30 mug Raji whole cell lysate/extract C. 30 mug NCI-H929 whole cell lysate/extract5 % SDS-PAGE diluted at 1:500)
Rabbit anti-Human BLM Polyclonal Antibody | anti-BLM antibody
BLM Antibody
Immunofluorescence: 1:100-1:1000
Testing Data
(BLM antibody detects BLM protein by western blot analysis.A. 30 mug Jurkat whole cell lysate/extract B. 30 mug Raji whole cell lysate/extract C. 30 mug NCI-H929 whole cell lysate/extract5 % SDS-PAGE diluted at 1:500)
Testing Data
(BLM antibody detects BLM protein by western blot analysis.A. 30 mug Jurkat whole cell lysate/extract B. 30 mug Raji whole cell lysate/extract C. 30 mug NCI-H929 whole cell lysate/extract5 % SDS-PAGE diluted at 1:500)
Testing Data
(BLM antibody, N-term detects BLM protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BLM protein stained by BLM antibody, N-term () diluted at 1:500.Blue: Hoechst 33342 staining.)
Testing Data
(BLM antibody, N-term detects BLM protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BLM protein stained by BLM antibody, N-term () diluted at 1:500.Blue: Hoechst 33342 staining.)
NCBI and Uniprot Product Information
NCBI Description
The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq, Jul 2008]
Uniprot Description
BLM: a magnesium-dependent ATP-dependent DNA-helicase that unwinds single- and double-stranded DNA in a 3'-5' direction. A member of the RecQ helicase family that is required for genome stability. Participates in DNA replication, recombination and repair. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex which is a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ubiquitinated FANCD2.
Protein type: DNA replication; EC 3.6.4.12; DNA repair, damage; Helicase
Chromosomal Location of Human Ortholog: 15q26.1
Cellular Component: male germ cell nucleus; lateral element; nuclear chromosome; chromosome, telomeric region; PML body; nuclear matrix; pronucleus; cytoplasm; nucleolus; replication fork; nucleus
Molecular Function: G-quadruplex DNA binding; four-way junction helicase activity; ATP-dependent DNA helicase activity; protein binding; ATP-dependent 3'-5' DNA helicase activity; p53 binding; ATPase activity; ATP-dependent helicase activity; bubble DNA binding; helicase activity; single-stranded DNA binding; ATP binding
Biological Process: negative regulation of DNA recombination; regulation of binding; positive regulation of transcription, DNA-dependent; DNA double-strand break processing; negative regulation of mitotic recombination; alpha-beta T cell differentiation; negative regulation of cell division; DNA repair; protein oligomerization; DNA duplex unwinding; double-strand break repair via homologous recombination; DNA recombination; replication fork processing; mitotic cell cycle G2/M transition DNA damage checkpoint; DNA strand renaturation; replication fork protection; positive regulation of alpha-beta T cell proliferation; regulation of cyclin-dependent protein kinase activity; telomere maintenance; response to DNA damage stimulus; response to X-ray
Disease: Bloom Syndrome
Research Articles on BLM
Similar Products
Product Notes
The BLM blm (Catalog #AAA9403090) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BLM Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BLM can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB). Western blotting: 1:500-1:3000 Immunofluorescence: 1:100-1:1000. Researchers should empirically determine the suitability of the BLM blm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BLM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
