Rabbit TRIAD3A Polyclonal Antibody | anti-TRIAD3A antibody

RABBIT ANTI MOUSE TRIAD3A (N-TERMINAL)

Cat. Num. AAA221309

• Gene Names: Rnf216; UIP83; C86502; TRIAD3; AI647468; AU019462; Ubce7ip1; 2810055G22Rik; F830018F18Rik
• Applications: Immunohistochemistry, Western Blot
• Synonyms: TRIAD3A; Polyclonal Antibody; RABBIT ANTI MOUSE TRIAD3A (N-TERMINAL); anti-TRIAD3A antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises an epitope within the N-terminal region (NT) of mouse TRIAD3A, a ubiquitously expressed E3 ubiquitin-protein ligase which plays an important role in the innate immune response. ; ; Regulation of the level and duration of Toll-like receptor (TLR) activation is vital in controlling a pro-inflammatory response, to prevent harm to the host. Several proteins involved in the regulation of TLR signaling have been described including ST2 and IL-1R-associated kinase M, along with many other adaptor proteins of the MyD88 family, which mediate TLR signal transduction. TRIAD3A, identified as a RING finger member of the E3 ligase family of proteins, binds to the cytoplasmic tail of several TLRs, notably TLR4 and TLR9, resulting in ubiquitination and proteolytic degradation, thereby regulating their expression. TRIAD3A does not appear to regulate the expression of TLR2.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 732

• Applicable Applications for anti-TRIAD3A antibody: Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 95kDa in mouse heart cell lysates.; Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide
• Immunogen: A 15 amino acid peptide located near mouse TRIAD3A amino-terminus.; Buffer Solution
• Antiserum Preparation: Antisera to mouse TRIAD3A (NT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of mouse heart lysate probed with Rabbit anti Mouse TRIAD3A (MBS221309) at 0.5(A), 1(B) and 2(C) ug/ml)

Testing Data #2

(Formalin fixed, paraffin embedded mouse heart stained with Rabbit anti Mouse TRIAD3A (MBS221309) following antigen retrieval using sodium citrate buffer (pH6.0))

Related Product Information for anti-TRIAD3A antibody

MBS221309 recognises an epitope within the N-terminal region (NT) of mouse TRIAD3A, a ubiquitously expressed E3 ubiquitin-protein ligase which plays an important role in the innate immune response. Regulation of the level and duration of Toll-like receptor (TLR) activation is vital in controlling a pro-inflammatory response, to prevent harm to the host. Several proteins involved in the regulation of TLR signaling have been described including ST2 and IL-1R-associated kinase M, along with many other adaptor proteins of the MyD88 family, which mediate TLR signal transduction. TRIAD3A, identified as a RING finger member of the E3 ligase family of proteins, binds to the cytoplasmic tail of several TLRs, notably TLR4 and TLR9, resulting in ubiquitination and proteolytic degradation, thereby regulating their expression. TRIAD3A does not appear to regulate the expression of TLR2.

NCBI and Uniprot Product Information

• NCBI GI #: 46358407
• NCBI GeneID: 108086
• NCBI Accession #: NP_542128.2
• NCBI GenBank Nucleotide #: NM_080561.4
• UniProt Accession #: P58283; Q3U493; Q3UE56; Q3UGM3; Q68FN0; Q6P1H8; Q6PWY5; Q8BN27; Q8C1U3
• Molecular Weight: 83,612 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF216 isoform A
• NCBI Official Synonym Full Names: ring finger protein 216
• NCBI Official Symbol: Rnf216
• NCBI Official Synonym Symbols: UIP83; C86502; TRIAD3; AI647468; AU019462; Ubce7ip1; 2810055G22Rik; F830018F18Rik
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF216; E3 ubiquitin ligase; triad domain-containing protein 3; ubcM4-interacting protein 83; ubiquitin-conjugating enzyme 7-interacting protein 1
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF216
• UniProt Gene Name: Rnf216
• UniProt Synonym Gene Names: Triad3; Ubce7ip1; Uip83; Zin
• UniProt Entry Name: RN216_MOUSE

Uniprot Description

TRIAD3: Isoform 1 acts as an E3 ubiquitin ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Promotes degradation of TRAF3, TLR4 and TLR9. Contributes to the regulation of antiviral responses. Down- regulates activation of NF-kappa-B, IRF3 activation and IFNB production. Isoform 3/ZIN inhibits TNF and IL-1 mediated activation of NF-kappa-B. Promotes TNF and RIP mediated apoptosis. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; EC 6.3.2.19; Ligase; Ubiquitin conjugating system; EC 6.3.2.-

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: zinc ion binding; coenzyme F420-2 alpha-glutamyl ligase activity; metal ion binding; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; ribosomal S6-glutamic acid ligase activity; coenzyme F420-0 gamma-glutamyl ligase activity; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; apoptosis; regulation of interferon-beta production; regulation of defense response to virus by host