Western Blot/Blotting
(Western blot analysis of TIRAP in MCF-7 cell lysate with TIRAP antibody (IN) at 4 g/ml.)
Rabbit anti-Human Toll Interleukin 1 receptor adaptor protein (TIRAP) Polyclonal Antibody | anti-TIRAP antibody
Toll Interleukin 1 receptor adaptor protein (TIRAP) (IN)
Gene Names
Tirap; Mal; Wyatt; Tlr4ap; AA407980; C130027E04Rik
Reactivity
Human
Applications
Western Blot
Purity
Antigen Immunoaffiinity Purification
Synonyms
Toll Interleukin 1 receptor adaptor protein (TIRAP); Polyclonal Antibody; Toll Interleukin 1 receptor adaptor protein (TIRAP) (IN); MyD88 adapter-like protein; Adaptor protein Wyatt; anti-TIRAP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Immunoaffiinity Purification
Form/Format
Provided as solution in phosphate buffered saline with 0.02% sodium azide
Sequence Length
249
Applicable Applications for anti-TIRAP antibody
Western Blot (WB)
Immunogen
Rabbit polyclonal TIRAP antibody was raised against a peptide corresponding to amino acids near the middle of human TIRAP.
Positive Control
Found in the cytoplasm and highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain.
Preparation and Storage
Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles
Western Blot/Blotting
(Western blot analysis of TIRAP in MCF-7 cell lysate with TIRAP antibody (IN) at 4 g/ml.)
Western Blot/Blotting
(Western blot analysis of TIRAP in MCF-7 cell lysate with TIRAP antibody (IN) at 4 g/ml.)
Related Product Information for anti-TIRAP antibody
Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1-3). These proteins act through adaptor molecules such as TIRAP and MyD88 to activate various kinases and transcription factors (4,5). In TIRAP-deficient mice, TLR signaling in response to TLR2 ligands (using either TLR1 and TLR6 as co-receptors) is totally abolished, suggesting that MyD88 and TIRAP work together and are both required for TLR2 signaling (6). Furthermore, these mice are also resistant to the toxic effects of LPS and show defects in NF-κB and MAP kinase activation, suggesting that TIRAP is also involed in TLR4 signaling (6,7)
Product Categories/Family for anti-TIRAP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26 kD
NCBI Official Full Name
toll/interleukin-1 receptor domain-containing adapter protein
NCBI Official Synonym Full Names
toll-interleukin 1 receptor (TIR) domain-containing adaptor protein
NCBI Official Symbol
Tirap
NCBI Official Synonym Symbols
Mal; Wyatt; Tlr4ap; AA407980; C130027E04Rik
NCBI Protein Information
toll/interleukin-1 receptor domain-containing adapter protein; adaptor protein Wyatt; myD88 adapter-like protein; Toll-like receptor 4 adaptor protein; TIR domain-containing adapter protein
UniProt Protein Name
Toll/interleukin-1 receptor domain-containing adapter protein
UniProt Gene Name
Tirap
UniProt Synonym Gene Names
Mal; TIR domain-containing adapter protein
UniProt Entry Name
TIRAP_MOUSE
Uniprot Description
Function: Adapter involved in the TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response
By similarity. Positively regulates the production of TNF-alpha and interleukin-6
By similarity.
Subunit structure: Homodimer. Also forms heterodimers with MYD88. Interacts with BMX and TBK1
By similarity. Interacts with TLR4 and IRAK2 via their respective TIR domains. Interacts with EIF2AK2. Does not interact with IRAK1-1, nor TLR9. May interact with PIK3AP1. Ref.4
Subcellular location: Cytoplasm
By similarity. Cell membrane
By similarity. Membrane
By similarity. Note: Colocalizes with DAB2IP at the plasma membrane
By similarity.
Post-translational modification: Phosphorylated by IRAK1 and IRAK4
By similarity. Also phosphorylated by BTK
By similarity.Polyubiquitinated. Polyubiquitination follows phosphorylation by BTK and leads to TIRAP degradation
By similarity.
Sequence similarities: Contains 1 TIR domain.
Research Articles on TIRAP
Similar Products
Product Notes
The TIRAP tirap (Catalog #AAA395622) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Toll Interleukin 1 receptor adaptor protein (TIRAP) (IN) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Toll Interleukin 1 receptor adaptor protein (TIRAP) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the TIRAP tirap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Toll Interleukin 1 receptor adaptor protein (TIRAP), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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