Rabbit anti-Mouse TRIAD3A Polyclonal Antibody | anti-TRAF3 antibody

TRIAD3A, NT (E3 Ubiquitin Ligase TRIAD3A, E3 ubiquitin-protein ligase RNF216, RING finger protein 216, Ubiquitin-conjugating enzyme 7-interacting protein 1, UbcM4-interacting protein 83, Triad domain-containing protein 3, Rnf216, Triad3, Ubce7ip1, Uip83,

Cat. Num. AAA647069

• Gene Names: TRAF3; CAP1; LAP1; CAP-1; CRAF1; IIAE5; CD40bp
• Reactivity: Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Highly Purified; Purified by Ion Exchange chromatography.
• Synonyms: TRIAD3A; Polyclonal Antibody; NT (E3 Ubiquitin Ligase TRIAD3A; E3 ubiquitin-protein ligase RNF216; RING finger protein 216; Ubiquitin-conjugating enzyme 7-interacting protein 1; UbcM4-interacting protein 83; Triad domain-containing protein 3; Rnf216; Triad3; Ubce7ip1; Uip83; ; Anti -TRIAD3A; anti-TRAF3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: Recognizes mouse TRIAD3A.
• Purity/Purification: Highly Purified; Purified by Ion Exchange chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-TRAF3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 0.5-2ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to 15aa from mouse TRIAD3A at N-terminal (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Testis (formalin-fixed, paraffin-embedded) stained with RNF216 antibody at 5ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-TRAF3 antibody

Activation of NF-kB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88. Several regulatory mechanisms exist to control TLR signal transduction, including the inhibition of TLR expression and signaling by molecules such as ST2 and SIGIRR. Another mechanism is by the ubiquitinization of selected TLRs by TRIAD3A, an E3 ubiquitin-protein ligase. TRIAD3A is a RING finger protein that can bind to TLR4 and TLR9, and to a lesser extent TLR3 and TLR5, catalyzing the ubiquitization of these molecules. Overexpression of TRIAD3A promoted the nearly complete degradation of TLR4 and TLR9; this reduction was reflected in the decreased signal-specific activation by ligands specific for these TLRs. Conversely, depletion of TRIAD3A resulted in enhanced TLR activation.

Product Categories/Family for anti-TRAF3 antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 116242824
• NCBI GeneID: 7187
• UniProt Accession #: Q13114; Q12990; Q13076; Q13947; Q6AZX1; Q9UNL1; B7Z8C4
• Molecular Weight: 64,490 Da
• NCBI Official Full Name: TNF receptor-associated factor 3
• NCBI Official Synonym Full Names: TNF receptor-associated factor 3
• NCBI Official Symbol: TRAF3
• NCBI Official Synonym Symbols: CAP1; LAP1; CAP-1; CRAF1; IIAE5; CD40bp
• NCBI Protein Information: TNF receptor-associated factor 3; CD40 binding protein; CD40 associated protein 1; LMP1-associated protein 1; CD40 receptor associated factor 1
• UniProt Protein Name: TNF receptor-associated factor 3
• Protein Family: TNF receptor-associated factor
• UniProt Gene Name: TRAF3
• UniProt Synonym Gene Names: CAP1; CRAF1; CRAF1; CD40BP; LAP1
• UniProt Entry Name: TRAF3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from, members of the TNF receptor (TNFR) superfamily. This protein participates in the signal transduction of CD40, a TNFR family member important for the activation of the immune response. This protein is found to be a critical component of the lymphotoxin-beta receptor (LTbetaR) signaling complex, which induces NF-kappaB activation and cell death initiated by LTbeta ligation. Epstein-Barr virus encoded latent infection membrane protein-1 (LMP1) can interact with this and several other members of the TRAF family, which may be essential for the oncogenic effects of LMP1. Several alternatively spliced transcript variants encoding three distinct isoforms have been reported. [provided by RefSeq, Dec 2010]

Uniprot Description

Function: Regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of B-cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa-B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14. Ref.27 Ref.28 Ref.29 Ref.33 Ref.34 Ref.35

Subunit structure: Homotrimer. Heterotrimer with TRAF2 and TRAF5. Interacts with LTBR/TNFRSF3, TNFRSF4, TNFRSF5/CD40, TNFRSF8/CD30, TNFRSF13C TNFRSF17/BCMA, TLR4 and EDAR. Interacts with MAP3K5, MAP3K14, TRAIP/TRIP, TDP2/TTRAP, TANK/ITRAF and TRAF3IP1. Interaction with TNFRSF5/CD40 is modulated by TANK/ITRAF, which competes for the same binding site. Interacts with TICAM1. Interacts with TRAFD1. Interacts with OTUB1, OTUB2 and OTUD5. Interacts with RNF216, MAVS, OPTN and TBK1. Identified in a complex with TRAF2, MAP3K14 and BIRC3. Interacts with BIRC2 and BIRC3. Upon exposure to bacterial lipopolysaccharide (LPS), recruited to a transient complex containing TLR4, TRAF3, TRAF6, IKBKG, MAP3K7, MYD88, TICAM1, BIRC2, BIRC3 and UBE2N

By similarity. Interacts with Epstein-Barr virus protein LMP1. Interacts (via RING-type zinc finger domain) with SRC. Interacts with CARD14. Ref.1 Ref.2 Ref.3 Ref.4 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Ref.23 Ref.24 Ref.25 Ref.26 Ref.27 Ref.28 Ref.29 Ref.30 Ref.31 Ref.32 Ref.33 Ref.36 Ref.37 Ref.40 Ref.41 Ref.42

Subcellular location: Cytoplasm

Probable. Endosome

By similarity. Mitochondrion. Note: Undergoes endocytosis together with TLR4 upon LPS signaling

By similarity. Associated with mitochondria in response to virus. Ref.27 Ref.33

Domain: The MATH/TRAF domain binds to receptor cytoplasmic domains.The Ring-type zinc finger domain is required for its function in down-regulation of NFKB2 proteolytic processing

By similarity.

Post-translational modification: Undergoes 'Lys-48'-linked polyubiquitination, leading to its proteasomal degradation in response to signaling by TNFSF13B, TLR4 or through CD40. 'Lys-48'-linked polyubiquitinated form is deubiquitinated by OTUD7B, preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappa-B. Undergoes 'Lys-63'-linked ubiquitination during early stages of virus infection, and 'Lys-48'-linked ubiquitination during later stages. Undergoes both 'Lys-48'-linked and 'Lys-63'-linked ubiquitination in response to TLR3 and TLR4 signaling. Deubiquitinated by OTUB1, OTUB2 and OTUD5.

Involvement in disease: Herpes simplex encephalitis 3 (HSE3) [MIM:614849]: A rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Ref.44

Sequence similarities: Belongs to the TNF receptor-associated factor family. A subfamily.Contains 1 MATH domain.Contains 1 RING-type zinc finger.Contains 2 TRAF-type zinc fingers.