Western Blot (WB)
((1ug/ml) staining of Olfactory bulb lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Synaptotagmin-5 Polyclonal Antibody | anti-Syt5 antibody
Goat anti-Synaptotagmin-5 (aa 80-93) Antibody
Gene Names
Syt5; SytV
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Dog, Pig, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Synaptotagmin-5; Polyclonal Antibody; Goat anti-Synaptotagmin-5 (aa 80-93) Antibody; Syt5; synaptotagmin V; SytV; synaptotagmin 5; synaptotagmin IX; synaptotagmin-5; synaptotagmin 5 antibody; synaptotagmin IX antibody; synaptotagmin V antibody; synaptotagmin-5 antibody; Syt5 antibody; SytV antibody; Synaptotagmin-5 (aa 80-93); anti-Syt5 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
SYIDKVQPEIEELD
Sequence Length
386
Applicable Applications for anti-Syt5 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 45kDa band observed in Human Brain (Olfactory bulb) lysates (calculated MW of 43.1kDa according to NP_058604.1). Recommended concentration: 1-3ug/ml.
Western Blot: Approx 45kDa band observed in Human Brain (Olfactory bulb) lysates (calculated MW of 43.1kDa according to NP_058604.1). Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence C-SYIDKVQPEIEELD, from the internal region of the protein sequence according to NP_058604.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((1ug/ml) staining of Olfactory bulb lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Olfactory bulb lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,130 Da
NCBI Official Full Name
synaptotagmin-5
NCBI Official Synonym Full Names
synaptotagmin V
NCBI Official Symbol
Syt5
NCBI Official Synonym Symbols
SytV
NCBI Protein Information
synaptotagmin-5
UniProt Protein Name
Synaptotagmin-5
Protein Family
UniProt Gene Name
Syt5
UniProt Synonym Gene Names
; SytV
UniProt Entry Name
SYT5_MOUSE
Uniprot Description
SYT5: May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Regulates the Ca(2+)- dependent secretion of norepinephrine in PC12 cells. Required for export from the endocytic recycling compartment to the cell surface. Belongs to the synaptotagmin family.
Protein type: Membrane protein, integral
Cellular Component: cell junction; cell soma; cytoplasmic vesicle; endosome; integral to membrane; membrane; neuron projection; perinuclear region of cytoplasm; plasma membrane; synaptic vesicle membrane
Molecular Function: calcium ion binding; calcium-dependent phospholipid binding; clathrin binding; metal ion binding; protein heterodimerization activity; syntaxin binding
Biological Process: calcium ion-dependent exocytosis of neurotransmitter; regulation of calcium ion-dependent exocytosis; synaptic vesicle endocytosis; vesicle fusion
Research Articles on Syt5
Similar Products
Product Notes
The Syt5 syt5 (Catalog #AAA422984) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Synaptotagmin-5 (aa 80-93) Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Synaptotagmin-5 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 45kDa band observed in Human Brain (Olfactory bulb) lysates (calculated MW of 43.1kDa according to NP_058604.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the Syt5 syt5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SYIDKVQPEI EELD. It is sometimes possible for the material contained within the vial of "Synaptotagmin-5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
