Western Blot (WB)
((1ug/ml) staining of MCF7 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Coilin Polyclonal Antibody | anti-COIL antibody
Goat anti-Coilin (aa73-86) Antibody
Western Blot: Approx 80kDa band observed in nuclear lysates of cell line MCF7 (calculated MW of 62.6kDa according to NP_004636.1). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((1ug/ml) staining of MCF7 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of MCF7 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14. [provided by RefSeq, Jul 2008]
Uniprot Description
coilin: an ubiquitous protein found in nuclear coiled bodies (CBS), nuclear suborganelles involved in the post-transcriptional modification of snRNPs and small nucleolar RNAs. snRNPs undergo 2'-O-methylation and pseudouridylation in CBS. Differential methylation and phosphorylation of coilin likely influences localization among nuclear bodies and the composition and assembly of CBS. Located in the interchromatin space between the nucleolus and the nucleus.
Protein type: Oxidoreductase
Chromosomal Location of Human Ortholog: 17q22
Cellular Component: Cajal body; membrane; nucleolus; nucleoplasm; nucleus
Molecular Function: identical protein binding; protein binding; protein C-terminus binding
Research Articles on COIL
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Product Notes
The COIL coil (Catalog #AAA423619) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Coilin (aa73-86) Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Rat, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Coilin can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 80kDa band observed in nuclear lysates of cell line MCF7 (calculated MW of 62.6kDa according to NP_004636.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the COIL coil for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RLVRDNDCLR VKLE. It is sometimes possible for the material contained within the vial of "Coilin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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