Western Blot (WB)
((1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat RARS Polyclonal Antibody | anti-RARS antibody
Goat anti-RARS Antibody
Western Blot: Approx 75kDa band observed in lysates of HeLa and HepG2 (calculated MW of 75.4kDa according to NP_002878.2). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Arginyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq, Jul 2008]
Uniprot Description
RARS: Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1. Belongs to the class-I aminoacyl-tRNA synthetase family. 2 isoforms of the human protein are produced by alternative initiation.
Protein type: Mitochondrial; Translation; EC 6.1.1.19; RNA-binding; Ligase
Chromosomal Location of Human Ortholog: 5q35.1
Cellular Component: aminoacyl-tRNA synthetase multienzyme complex; cytoplasm; cytosol; membrane; mitochondrion; nucleoplasm
Molecular Function: arginine-tRNA ligase activity; ATP binding; protein binding; tRNA binding
Biological Process: arginyl-tRNA aminoacylation; tRNA aminoacylation for protein translation
Disease: Leukodystrophy, Hypomyelinating, 9
Research Articles on RARS
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Product Notes
The RARS rars (Catalog #AAA421905) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-RARS Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's RARS can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 75kDa band observed in lysates of HeLa and HepG2 (calculated MW of 75.4kDa according to NP_002878.2). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the RARS rars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SLQAERNKPT KN. It is sometimes possible for the material contained within the vial of "RARS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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