Western Blot (WB)
((2ug/ml) staining of Rat Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Prdm9 Polyclonal Antibody | anti-Prdm9 antibody
Goat anti-Prdm9 (rat aa440-451) Antibody
Gene Names
Prdm9; Prdm7
Reactivity
Tested: Rat; Expected from sequence similarity: Rat
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Prdm9; Polyclonal Antibody; Goat anti-Prdm9 (rat aa440-451) Antibody; PR domain containing 9; Prdm7; PR domain containing 7; PR domain zinc finger protein 9; PR domain-containing protein 9; histone-lysine N-methyltransferase PRDM9; Prdm9 antibody; PR domain containing 9 antibody; Prdm7 antibody; PR domain containing 7 antibody; PR domain zinc finger protein 9 antibody; PR domain-containing protein 9 antibody; histone-lysine N-methyltransferase PRDM9 antibody; Prdm9 (rat aa440-451); anti-Prdm9 antibody
Host
Goat
Reactivity
Tested: Rat; Expected from sequence similarity: Rat
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
QEHFDSQNKNDK
Sequence Length
796
Applicable Applications for anti-Prdm9 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 90kDa band observed in Rat Ovary lysates (calculated MW of 92.6kDa according to NP_001102373.2). Recommended concentration: 2-3ug/ml.
Western Blot: Approx 90kDa band observed in Rat Ovary lysates (calculated MW of 92.6kDa according to NP_001102373.2). Recommended concentration: 2-3ug/ml.
Immunogen
Peptide with sequence C-QEHFDSQNKNDK, from the internal region of the protein sequence according to NP_001102373.2.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((2ug/ml) staining of Rat Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((2ug/ml) staining of Rat Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,406 Da
NCBI Official Full Name
histone-lysine N-methyltransferase PRDM9
NCBI Official Synonym Full Names
PR domain 9
NCBI Official Symbol
Prdm9
NCBI Official Synonym Symbols
Prdm7
NCBI Protein Information
histone-lysine N-methyltransferase PRDM9
UniProt Protein Name
Histone-lysine N-methyltransferase PRDM9
Protein Family
UniProt Gene Name
Prdm9
UniProt Entry Name
PRDM9_RAT
Uniprot Description
Histone methyltransferase that specifically trimethylates 'Lys-4' of histone H3 during meiotic prophase and is essential for proper meiotic progression. Does not have the ability to mono- and dimethylate 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes during early meiotic prophase ().
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Product Notes
The Prdm9 prdm9 (Catalog #AAA423423) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Prdm9 (rat aa440-451) Antibody reacts with Tested: Rat; Expected from sequence similarity: Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Prdm9 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 90kDa band observed in Rat Ovary lysates (calculated MW of 92.6kDa according to NP_001102373.2). Recommended concentration: 2-3ug/ml. Researchers should empirically determine the suitability of the Prdm9 prdm9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QEHFDSQNKN DK. It is sometimes possible for the material contained within the vial of "Prdm9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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