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Western Blot (WB) ((1ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat GLP-1-R Polyclonal Antibody | anti-Glp1r antibody

Goat anti-GLP-1-R (mouse) Antibody

Gene Names
Glp1r; GLP-1R; GLP1Rc
Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
GLP-1-R; Polyclonal Antibody; Goat anti-GLP-1-R (mouse) Antibody; Glp1r; glucagon-like peptide 1 receptor; GLP-1R; GLP1Rc; GLP-1 receptor; GLP-1 receptor antibody; Glp1r antibody; GLP-1R antibody; GLP-1-R antibody; GLP1Rc antibody; glucagon-like peptide 1 receptor antibody; GLP-1-R (mouse); anti-Glp1r antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
SKRGERNFPEEQ
Sequence Length
463
Applicable Applications for anti-Glp1r antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 55kDa band observed in Mouse Heart lysates (calculated MW of 53. 0kDa according to NP_067307.2). Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence C-SKRGERNFPEEQ, from the internal region of the protein sequence according to NP_067307.2.
Epitope
Internal region
Note
The immunizing peptide represents part of an extracellular region.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((1ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB)

((1ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((1ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,028 Da
NCBI Official Full Name
glucagon-like peptide 1 receptor
NCBI Official Synonym Full Names
glucagon-like peptide 1 receptor
NCBI Official Symbol
Glp1r
NCBI Official Synonym Symbols
GLP-1R; GLP1Rc
NCBI Protein Information
glucagon-like peptide 1 receptor
UniProt Protein Name
Glucagon-like peptide 1 receptor
UniProt Gene Name
Glp1r
UniProt Synonym Gene Names
GLP-1 receptor; GLP-1-R; GLP-1R
UniProt Entry Name
GLP1R_MOUSE

Uniprot Description

GLP1R: This is a receptor for glucagon-like peptide 1. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Belongs to the G-protein coupled receptor 2 family.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 2

Cellular Component: integral to membrane; integral to plasma membrane; intracellular; membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; glucagon receptor activity; peptide hormone binding; peptide receptor activity; peptide receptor activity, G-protein coupled; receptor activity; signal transducer activity; transmembrane receptor activity

Biological Process: associative learning; cAMP-mediated signaling; cell surface receptor linked signal transduction; elevation of cytosolic calcium ion concentration; feeding behavior; G-protein coupled receptor protein signaling pathway; G-protein signaling, adenylate cyclase activating pathway; hormone secretion; insulin secretion; learning and/or memory; memory; negative regulation of apoptosis; negative regulation of neuron apoptosis; neuropeptide signaling pathway; positive regulation of blood pressure; positive regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of heart contraction; positive regulation of insulin secretion; positive regulation of transcription from RNA polymerase II promoter; regulation of calcium ion transport; regulation of heart contraction; release of sequestered calcium ion into cytosol; response to glucose stimulus; response to stress; signal transduction

Research Articles on Glp1r

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Product Notes

The Glp1r glp1r (Catalog #AAA423129) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-GLP-1-R (mouse) Antibody reacts with Tested: Mouse; Expected from sequence similarity: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GLP-1-R can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 55kDa band observed in Mouse Heart lysates (calculated MW of 53. 0kDa according to NP_067307.2). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the Glp1r glp1r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SKRGERNFPE EQ. It is sometimes possible for the material contained within the vial of "GLP-1-R, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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