Western Blot (WB)
((0. 01ug/ml) staining of Human (A, C) and Mouse (B, D) Skeletal Muscle (A, B) and Heart (C, D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat creatine kinase M-type Polyclonal Antibody | anti-CKM antibody
Goat anti-creatine kinase M-type Antibody
Western Blot: Approx 45kDa band observed in Human and Mouse Skeletal Muscle and Heart lysates (calculated MW of 43.1kDa according to NP_001815.2). Recommended concentration: 0.01-0.03ug/ml.
Western Blot (WB)
((0. 01ug/ml) staining of Human (A, C) and Mouse (B, D) Skeletal Muscle (A, B) and Heart (C, D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 01ug/ml) staining of Human (A, C) and Mouse (B, D) Skeletal Muscle (A, B) and Heart (C, D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]
Uniprot Description
CKM: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.
Protein type: EC 2.7.3.2; Kinase, other; Amino Acid Metabolism - arginine and proline
Chromosomal Location of Human Ortholog: 19q13.32
Cellular Component: cytosol
Molecular Function: ATP binding; creatine kinase activity; protein binding
Biological Process: creatine metabolic process; phosphocreatine biosynthetic process; phosphorylation
Research Articles on CKM
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Product Notes
The CKM ckm (Catalog #AAA423019) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-creatine kinase M-type Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's creatine kinase M-type can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 45kDa band observed in Human and Mouse Skeletal Muscle and Heart lysates (calculated MW of 43.1kDa according to NP_001815.2). Recommended concentration: 0.01-0.03ug/ml. Researchers should empirically determine the suitability of the CKM ckm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NTHNKFKLNY KPEE. It is sometimes possible for the material contained within the vial of "creatine kinase M-type, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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