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Testing Data

Rabbit anti-Human SLIT-3 Polyclonal Antibody | anti-SLIT-3 antibody

Anti-SLIT-3 Antibody

Reactivity
Human
Applications
Western Blot, ELISA
Purity
Affinity Purified
Synonyms
SLIT-3; Polyclonal Antibody; Anti-SLIT-3 Antibody; anti-SLIT-3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
This affinity purified antibody is directed against human SLIT-3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, rat, dog, bovine and mouse based on 100% homology for the immunogen sequence. Expect cross reactivity with SLIT-3 from chimpanzee and chicken sources, as only a single amino acid residue change is found within the immunogen sequence (93% positive by BLAST). Cross reactivity with SLIT-3 homologues from other sources has not been determined.
Purity/Purification
Affinity Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Sequence Length
1523
Applicable Applications for anti-SLIT-3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~ 168 kDa corresponding to full length protein as well as multiple bands corresponding to isoforms of SLIT-3 by western blotting in the appropriate cell lysate or extract.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 1164-1177 of Human SLIT-3 protein.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Testing Data
Related Product Information for anti-SLIT-3 antibody
SLIT-3 (also known as multiple epidermal growth factorlike domain 5 and Slit homolog 3 protein) is a Slit protein. The 'slit' gene has been shown to play a critical role in central nervous system midline formation. In addition to SLIT3 there are two additional human 'slit' homologs, which are termed SLIT1 and SLIT2. Each SLIT gene encodes a putative secreted protein, which contains conserved protein-protein interaction domains including leucine-rich repeats and epidermal growth factor-like motifs, similar to those of the Drosophila protein. SLIT proteins may also participate in the formation and maintenance of the nervous and endocrine systems by protein-protein interactions. Slit-3 is a secreted protein predominantly expressed in thyroid. Multiple isoforms have been reported for this product.

NCBI and Uniprot Product Information

NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
slit homolog 3 protein isoform 2

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Product Notes

The SLIT-3 (Catalog #AAA4158689) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SLIT-3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SLIT-3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~ 168 kDa corresponding to full length protein as well as multiple bands corresponding to isoforms of SLIT-3 by western blotting in the appropriate cell lysate or extract. Researchers should empirically determine the suitability of the SLIT-3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLIT-3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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