Rabbit anti-Mouse SLIT-1 Polyclonal Antibody | anti-SLIT-1 antibody

Anti-SLIT-1 Antibody

Cat. Num. AAA4158687

• Gene Names: SLIT1; MEGF4; SLIL1; SLIT3; SLIT-1
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Affinity Purified
• Synonyms: SLIT-1; Polyclonal Antibody; Anti-SLIT-1 Antibody; anti-SLIT-1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This affinity purified antibody is directed against mouse SLIT-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse and rat based on 100% homology for the immunogen sequence. Expect cross reactivity with SLIT-1 from human, chicken and frog sources, as only a single amino acid residue change is found within the immunogen sequence (94% positive by BLAST). Cross reactivity with SLIT-1 homologues from other sources has not been determined.
• Purity/Purification: Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 1534

• Applicable Applications for anti-SLIT-1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect multiple bands corresponding to cleavage products_recover of SLIT-1 by western blotting in the appropriate cell lysate or extract.
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 487-504 of mouse SLIT-1 protein.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-SLIT-1 antibody

SLIT-1 (also known as KIAA0813, MEGF4, multiple epidermal growth factor-like domains 4 and Slit homolog 1 protein) is a Slit protein. This protein is a ligand for the Roundabout (Robo) receptors and acts as guidance cues in axonal migration/navigation during neural development, at the ventral midline of the neural tube. Slit1 and Slit2 are essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb. Each SLIT gene encodes a putative secreted protein, which contains conserved protein-protein interaction domains including leucine-rich repeats and epidermal growth factor-like motifs, similar to those of the Drosophila protein. In situ hybridization studies indicated that the rat SLIT-1 mRNA was specifically expressed in the neurons of fetal and adult forebrains. This data suggests that the SLIT genes form an evolutionarily conserved group in vertebrates and invertebrates, and that the mammalian SLIT proteins may participate in the formation and maintenance of the nervous and endocrine systems by protein-protein interactions. Alternative splicing isoforms have been identified for Slit1 protein.

NCBI and Uniprot Product Information

• NCBI GI #: 188528675
• NCBI GeneID: 6585
• NCBI GenBank Nucleotide #: NP_003052.2
• UniProt Accession #: O75093
• NCBI Official Full Name: slit homolog 1 protein
• NCBI Official Synonym Full Names: slit guidance ligand 1
• NCBI Official Symbol: SLIT1
• NCBI Official Synonym Symbols: MEGF4; SLIL1; SLIT3; SLIT-1
• NCBI Protein Information: slit homolog 1 protein
• UniProt Protein Name: Slit homolog 1 protein
• UniProt Gene Name: SLIT1
• UniProt Synonym Gene Names: KIAA0813; MEGF4; SLIL1; Slit-1; Multiple EGF-like domains protein 4

Uniprot Description

Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.