Rabbit anti-Human, Rat CDC27 Polyclonal Antibody | anti-CDC27 antibody

Anti-CDC27 Antibody

Cat. Num. AAA4156618

• Gene Names: CDC27; APC3; HNUC; NUC2; H-NUC; ANAPC3; CDC27Hs; D0S1430E; D17S978E
• Reactivity: Human, Rat
• Applications: Western Blot, ELISA
• Purity: Affinity Purified
• Synonyms: CDC27; Polyclonal Antibody; Anti-CDC27 Antibody; anti-CDC27 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This affinity purified antibody is directed against human Cdc27 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, chicken, rat and dog based on 100% homology for the immunogen sequence. Expect cross reactivity with cdc27 from mouse as only a single amino acid residue change is found for the immunogen sequence. Cross reactivity with cdc27 homologues from other sources has not been determined.
• Purity/Purification: Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 823

• Applicable Applications for anti-CDC27 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 92 kDa in size corresponding to Cdc27 by western blotting in the appropriate cell lysate or extract.
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids 422-430 of Human cdc27.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-CDC27 antibody

Cdc27 (also known as cell division cycle protein 27 homologue, CDC27Hs and H-NUC) shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. Cdc27 is a component of anaphasepromoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Cdc27 has a nuclear localization.

NCBI and Uniprot Product Information

• NCBI GI #: 405833
• NCBI GeneID: 996
• NCBI Official Full Name: CDC27
• NCBI Official Synonym Full Names: cell division cycle 27
• NCBI Official Symbol: CDC27
• NCBI Official Synonym Symbols: APC3; HNUC; NUC2; H-NUC; ANAPC3; CDC27Hs; D0S1430E; D17S978E
• NCBI Protein Information: cell division cycle protein 27 homolog
• Protein Family: Cell division cycle protein

NCBI Description

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y. [provided by RefSeq, May 2014]