Rabbit NRF1 Polyclonal Antibody | anti-NRF1 antibody

Anti-NRF1 Antibody

Cat. Num. AAA4156781

• Gene Names: NRF1; ALPHA-PAL
• Reactivity: Human, Mouse, Rat, Avian
• Applications: Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: NRF1; Polyclonal Antibody; Anti-NRF1 Antibody; anti-NRF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Avian
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This protein A purified antibody is directed against mouse NRF1. The product was purified from monospecific antiserum by protein A affinity purification. BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, rat, chicken, frog and zebrafish based on very high sequence homology with the immunogen sequence. Cross reactivity with NRF1 homologues from other sources has not been determined.
• Purity/Purification: Protein A Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 522

• Applicable Applications for anti-NRF1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: This protein A purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 67 kDa in size corresponding to NRF1 by western blotting in the appropriate cell lysate or extract. Splice variants exist for This protein that may result in the detection of lower molecular weight bands.
• Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant mouse NRF1 protein corresponding to aa 1-534 of the native protein.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Testing Data

Related Product Information for anti-NRF1 antibody

NRF1 (also known as nuclear respiratory factor 1, alpha palindromic binding protein and alpha-pal) is the mammalian homolog to the erect wing (ewg) Drosophila protein that is required for proper development of the central nervous system and indirect flight muscles. In mammals NRF1 functions as a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. This protein links the transcriptional modulation of key metabolic genes to cellular growth and development and has been implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. NRF1 forms a homodimer and binds DNA as a dimer. NRF1 shows a nuclear localization and is widely expressed in embryonic, fetal, and adult tissues. Phosphorylation of NRF1 enhances DNA binding. Multiple splice variants have been identified for this protein.

NCBI and Uniprot Product Information

• NCBI GI #: 16877341
• NCBI GeneID: 4899
• NCBI Official Full Name: NRF1 protein
• NCBI Official Synonym Full Names: nuclear respiratory factor 1
• NCBI Official Symbol: NRF1
• NCBI Official Synonym Symbols: ALPHA-PAL
• NCBI Protein Information: nuclear respiratory factor 1
• Protein Family: Nuclear respiratory factor

NCBI Description

This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014]