Mouse Enteropeptidase ELISA Kit | Tmprss15 elisa kit

Mouse Enteropeptidase ELISA Kit

Cat. Num. AAA288144

• Gene Names: Tmprss15; Entk; Prss7
• Reactivity: Mouse
• Synonyms: Enteropeptidase; Mouse Enteropeptidase ELISA Kit; Tmprss15 elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse Enteropeptidase. No significant cross-reactivity or interference was observed.
• Sequence Length: 1019

• Assay Type: Sandwich
• Detection Range: 78-5000 pg/ml
• Sensitivity: 40 pg/mL
• Intra-Assay CV: <=7.2%
• Inter-Assay CV: <=9.5%
• Recovery: 84%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Tmprss15 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Enteropeptidase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Enteropeptidase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Enteropeptidase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Enteropeptidase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6679489
• NCBI GeneID: 19146
• NCBI Accession #: NP_032967.1
• NCBI GenBank Nucleotide #: NM_008941.3
• UniProt Accession #: P97435; Q148Y3
• Molecular Weight: 118,735 Da
• NCBI Official Full Name: enteropeptidase isoform 1
• NCBI Official Synonym Full Names: transmembrane protease, serine 15
• NCBI Official Symbol: Tmprss15
• NCBI Official Synonym Symbols: Entk; Prss7
• NCBI Protein Information: enteropeptidase
• UniProt Protein Name: Enteropeptidase
• Protein Family: Enteropeptidase
• UniProt Gene Name: Tmprss15
• UniProt Synonym Gene Names: Entk; Prss7
• UniProt Entry Name: ENTK_MOUSE

NCBI Description

This gene encodes an enzyme that proteolytically activates the pancreatic proenzyme trypsinogen, converting it into trypsin. The encoded protein is cleaved into two chains that form a heterodimer linked by a disulfide bond. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]

Uniprot Description

PRSS7: Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases. Defects in TMPRSS15 are a cause of enterokinase deficiency (ENTKD); a life-threatening intestinal malabsorption disorder characterized by diarrhea and failure to thrive. Belongs to the peptidase S1 family.

Protein type: Protease; EC 3.4.21.9; Membrane protein, integral

Cellular Component: integral to membrane; membrane

Molecular Function: hydrolase activity; peptidase activity; scavenger receptor activity; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: proteolysis