Western Blot (WB)
(Western blot analysis showing detection of 67 kDa 4-Hydroxynonenal BSA Conjugate using Anti-4-Hydroxynonenal Antibody, Clone 12F7 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 ug). Lane 3: BSA (1.0 ug). Lane 4: 4-Hydroxynonenal BSA (0.5 ug). Lane 5: 4-Hydroxynonenal BSA (1.0 ug). Block: 5% Skim Milk in 1X TBST. Primary Antibody: Anti-4-Hydroxynonenal Antibody, Clone 12F7 at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.)
4-Hydroxynonenal Protein | 4-HNE protein
4-Hydroxynonenal BSA Conjugate
Applications
Western Blot, ELISA
Purity
Filter Sterilized (0.2 um)
Synonyms
4-Hydroxynonenal; 4-Hydroxynonenal BSA Conjugate; Bovine Serum Albumin (BSA) modified with 4-Hydroxy nonenal (4-HNE); 4-Hydroxy Nonenal (4-HNE) BSA Conjugate; 4-Hydroxy Nonenal BSA Conjugate; 4-HNE BSA Conjugate; 4-Hydroxy Nonenal-BSA Conjugate; 4-Hydroxy Nonenal (BSA) Conjugate; MG-BSA; MG-BSA Conjugate; 4-Hydroxy Nonenal-modified BSA; 4-Hydroxy Nonenal Bovine Serum Albumin Conjugate; 4-Hydroxy Nonenal-Bovine Serum Albumin Conjugate; 4-Hydroxy Nonenal (Bovine Serum Albumin) Conjugate; 4-Hydroxy Nonenal-modified Bovine Serum Albumin; 4-Hydroxy Nonenal-bovine serum albumin; 4-hydroxy Hexenal BSA Conjugate; HNE BSA Conjugate; 4-hydroxy-2-nonenal BSA Conjugate; 4-HNE protein
Purity/Purification
Filter Sterilized (0.2 um)
Form/Format
PBS pH 7.4,0.09% Sodium Azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for 4-HNE protein
Western Blot (WB), ELISA (EIA)
Application Notes
Detectable in ELISA at > 5 ng.
Expression System
Synthetic
Tag Information
No tag
Research Areas
Cancer; Oxidative Stress; Lipid peruxidation.
Preparation and Storage
Store at -20°C for long term storage. Avoid freeze/ thaw cycle.
Western Blot (WB)
(Western blot analysis showing detection of 67 kDa 4-Hydroxynonenal BSA Conjugate using Anti-4-Hydroxynonenal Antibody, Clone 12F7 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 ug). Lane 3: BSA (1.0 ug). Lane 4: 4-Hydroxynonenal BSA (0.5 ug). Lane 5: 4-Hydroxynonenal BSA (1.0 ug). Block: 5% Skim Milk in 1X TBST. Primary Antibody: Anti-4-Hydroxynonenal Antibody, Clone 12F7 at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.)
Western Blot (WB)
(Western blot analysis showing detection of 67 kDa 4-Hydroxynonenal BSA Conjugate using Anti-4-Hydroxynonenal Antibody, Clone 12F7 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 ug). Lane 3: BSA (1.0 ug). Lane 4: 4-Hydroxynonenal BSA (0.5 ug). Lane 5: 4-Hydroxynonenal BSA (1.0 ug). Block: 5% Skim Milk in 1X TBST. Primary Antibody: Anti-4-Hydroxynonenal Antibody, Clone 12F7 at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.)
Product Categories/Family for 4-HNE protein
Similar Products
Product Notes
The 4-HNE (Catalog #AAA8001842) is a Protein and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is No tag!!Research Areas||Cancer; Oxidative Stress; Lipid peruxidation. AAA Biotech's 4-Hydroxynonenal can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Detectable in ELISA at > 5 ng. Researchers should empirically determine the suitability of the 4-HNE for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "4-Hydroxynonenal, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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