Testing Data
Rabbit SEK1/MKK4/JKK1 Polyclonal Antibody | anti-SEK1/MKK4/JKK1 antibody
SEK1 / MKK4 / JKK1 Antibody
Reactivity
Human, Mouse, Dog
Applications
Western Blot
Purity
Affinity purified
Synonyms
SEK1/MKK4/JKK1; Polyclonal Antibody; SEK1 / MKK4 / JKK1 Antibody; NKK; JNKK1; MAP kinase kinase 4; MAP2K4; MAPK/ERK kinase 4; MAPKK4; MEK4; monkeyK4; PRKMK4; SAPK/ERK kinase 1; SEK1; SERK1; anti-SEK1/MKK4/JKK1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Dog
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
100 ug (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Appearance: Colorless liquid
Appearance: Colorless liquid
Concentration
0.5 mg/ml (varies by lot)
Applicable Applications for anti-SEK1/MKK4/JKK1 antibody
Western Blot (WB)
Application Notes
Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes unphosphorylated and phosphorylated SEK1 from samples of human, mouse, and dog origins.
Immunogen
Synthetic peptide surrounding amino acid 347 of mouse SEK1
Positive Control
Western Blot: Jurkat cell lysate
Handling
The antibody solution should be gently mixed before use.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Testing Data
Testing Data
Related Product Information for anti-SEK1/MKK4/JKK1 antibody
Affinity purified polyclonal antibody to detect SEK1 (MKK4/JKK1) in human, mouse and dog samples.
Background: SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflammatory cytokines. Activation of SEK1 occurs thromugh phosphorylation of serine and threonine residues at positions 257 and 261, respectively, by MEKK. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop. Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses the stress-activated signal transduction.
Background: SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflammatory cytokines. Activation of SEK1 occurs thromugh phosphorylation of serine and threonine residues at positions 257 and 261, respectively, by MEKK. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop. Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses the stress-activated signal transduction.
Product Categories/Family for anti-SEK1/MKK4/JKK1 antibody
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Product Notes
The SEK1/MKK4/JKK1 (Catalog #AAA840407) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SEK1 / MKK4 / JKK1 Antibody reacts with Human, Mouse, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's SEK1/MKK4/JKK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes unphosphorylated and phosphorylated SEK1 from samples of human, mouse, and dog origins. Researchers should empirically determine the suitability of the SEK1/MKK4/JKK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SEK1/MKK4/JKK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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