FOLR2 recombinant protein

FOLR2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120742

• Gene Names: FOLR2; FBP; FR-P3; FR-BETA; BETA-HFR; FBP/PL-1
• Purity: >87% as determined by SDS-PAGE
• Synonyms: FOLR2; FOLR2 Protein; Human; Recombinant (His Tag); Human FOLR2/FBP Protein (His Tag); BETA-HFR Protein; FBP Protein; FBP/PL-1 Protein; FR-BETA Protein; FR-P3 Protein; folate receptor 2 (fetal); FOLR2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >87% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-His228

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Thr 17
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human FOLR2 (P14207-1) without the propeptide (Met 1-His 228) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for FOLR2 recombinant protein

Background: Folate receptor beta, also known as Folate receptor 2, FBP, and FOLR2, is a member of the folate receptor family. FOLR2 is expressed in placenta and hematopoietic cells. The expression of FOLR2 is increased in malignant tissues. Members of the Folate receptor family members (FOLRs) have a high affinity for folic acid and for several reduced folic acid derivatives. They mediate the delivery of 5-methyltetrahydrofolate to the interior of, out of within, or between cells in a process known as potocytosis. FOLR2 has a 68% and 79% sequence homology with the FOLR1 and FOLR3 proteins, respectively. The FOLR2 protein was originally thought to exist only in placenta, but is also detected in spleen, bone marrow, and thymus. FOLR2 is a marker for macrophages generated in the presence of M-CSF, but not GM-CSF. Its expression correlates with increased folate uptake ability. Folate conjugates of therapeutic drugs are a potential immunotherapy tool to target tumor-associated macrophages.

References

van Heyningen V, et al., 1995, Cytogenet Cell Genet. 69 (3-4): 127-58. Sabharanjak, S. et al., 2004, Adv Drug Deliv Rev. 56 (8): 1099-109. Scapoli, L. et al., 2005, Am J Med Genet A. 132A (3): 302-4. Puig-Kroger, A. et al., 2009, Cancer Res 69 (24): 9395-403.

NCBI and Uniprot Product Information

• NCBI GI #: 166064050
• NCBI GeneID: 2350
• NCBI Accession #: NP_000794.3
• NCBI GenBank Nucleotide #: NM_000803.4
• UniProt Accession #: P14207; Q05CA5; Q6GTE8
• Molecular Weight: 29,280 Da
• NCBI Official Full Name: folate receptor beta
• NCBI Official Synonym Full Names: folate receptor beta
• NCBI Official Symbol: FOLR2
• NCBI Official Synonym Symbols: FBP; FR-P3; FR-BETA; BETA-HFR; FBP/PL-1
• NCBI Protein Information: folate receptor beta
• UniProt Protein Name: Folate receptor beta
• Protein Family: Folate receptor
• UniProt Gene Name: FOLR2
• UniProt Synonym Gene Names: FR-beta; FBP

NCBI Description

The protein encoded by this gene is a member of the folate receptor (FOLR) family, and these genes exist in a cluster on chromosome 11. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and they mediate delivery of 5-methyltetrahydrofolate to the interior of cells. This protein has a 68% and 79% sequence homology with the FOLR1 and FOLR3 proteins, respectively. Although this protein was originally thought to be specific to placenta, it can also exist in other tissues, and it may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients. Multiple transcript variants that encode the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

FOLR2: Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. Belongs to the folate receptor family.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 11q13.4

Cellular Component: anchored to external side of plasma membrane; cell surface; membrane

Molecular Function: drug binding; folic acid binding; folic acid transporter activity; methotrexate binding

Biological Process: folic acid transport; monocyte chemotaxis; positive regulation of cell proliferation; receptor-mediated endocytosis; regulation of thymidylate synthase biosynthetic process