Rabbit anti-Human SEK1 Polyclonal Antibody | anti-Map2k4 antibody

SEK1, phosphorylated (Ser80) (MKK4, SAPK, Erk Kinase, MKK4, Jun Kinase Kinase, JNKK)

Cat. Num. AAA615479

• Gene Names: Map2k4; MEK4; MKK4; Sek1; JNKK1; Serk1; PRKMK4
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Synonyms: SEK1; Polyclonal Antibody; phosphorylated (Ser80) (MKK4; SAPK; Erk Kinase; MKK4; Jun Kinase Kinase; JNKK); Anti -SEK1; anti-Map2k4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of SEK1/MKK4 only when phosphorylated at serine 80. Does not crossreact with the corresponding phosphorylated residues of MEK1, MEK2 or MKK3.
• Purity/Purification: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.

• Applicable Applications for anti-Map2k4 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blotting, Immunoprecipitation.; Dilution: Western Blot: 1:1000; Immunoprecipitation 1:25
• Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser80 of human SEK1/MKK4.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from NIH/3T3 cells, untreated (lane 1), PDGF-treated (lane 2) or stably transfected with Src (lane 3), using Phospho-SEK1/MKK4 (Ser80) Antibody (upper) or SEK1/MKK4 Antibody (lower).)

Related Product Information for anti-Map2k4 antibody

SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflammatory cytokines (1-3). Activation of SEK1 occurs through phosphorylation of serine and threonine residues at positions 257 and 261, respectively, by MEKK. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop (4). Phosphorylation by Akt at serine 80 inhibits SEK1 and suppresses the stress-activated signal transduction (5).

Product Categories/Family for anti-Map2k4 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 1754531
• NCBI GeneID: 26398
• UniProt Accession #: P47809; Q543X6; Q8K2U0
• Molecular Weight: 44,114 Da
• NCBI Official Full Name: SEK1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 4
• NCBI Official Symbol: Map2k4
• NCBI Official Synonym Symbols: MEK4; MKK4; Sek1; JNKK1; Serk1; PRKMK4
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 4; MEK 4; JNKK 1; MAPKK 4; JNK kinase 1; MAPK/ERK kinase 4; SAPK/ERK kinase 1; SAPK/Erk/kinase 1; OTTMUSP00000006102; MAP kinase kinase 4; JNK-activating kinase 1; C-JUN N-terminal kinase kinase 1; mitogen activated protein kinase kinase 4, variant 1
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 4
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: Map2k4
• UniProt Synonym Gene Names: Jnkk1; Prkmk4; Sek1; Serk1
• UniProt Entry Name: MP2K4_MOUSE

Uniprot Description

MKK4: dual specificity kinase of the STE7 family that phosphorylates and activates JNK1 and -2 as well as p38 but not ERK1 or -2. Mediates cellular responses to various cellular stresses and inflammatory cytokines. Phosphorylation by Akt inhibits MKK4 and suppresses stress-activated signal transduction.

Protein type: Kinase, protein; EC 2.7.12.2; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); STE group; STE7 family

Cellular Component: dendrite cytoplasm; axon; cytoplasm; perikaryon; intracellular; cytosol; nucleus

Molecular Function: transferase activity; MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; mitogen-activated protein kinase kinase kinase binding; JUN kinase kinase activity; ATP binding; protein kinase activity

Biological Process: positive regulation of apoptosis; apoptosis; positive regulation of neuron apoptosis; MAPKKK cascade; JNK cascade; positive regulation of protein amino acid phosphorylation; protein amino acid phosphorylation; phosphorylation; positive regulation of DNA replication; activation of JNK activity; positive regulation of nitric-oxide synthase biosynthetic process