Mouse MKK4 Monoclonal Antibody | anti-MKK4 antibody

MKK4 (N-terminal region) Antibody

Cat. Num. AAA474443

• Gene Names: MAP2K4; JNKK; MEK4; MKK4; SEK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA
• Synonyms: MKK4; Monoclonal Antibody; MKK4 (N-terminal region) Antibody; MKK4 (N-terminal region); anti-MKK4 antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: M336
• Specificity: This antibody detects a 44kDa* protein corresponding to the molecular mass of MKK4 on SDS-PAGE immunoblots of adult mouse brain.
• Form/Format: Mouse monoclonal, protein A purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-MKK4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA: 1:2000; WB: 1:1000
• Immunogen: Clone (M336) was generated from a sequence corresponding to amino acids in the N-terminal region of the human MKK4. This sequence has high homology to rat and mouse MKK4.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot of mouse brain lysate. The blot was probed with mouse monoclonal anti-MKK4 (N-terminal region) antibody at 1:250 (lane 1), 1:500 (lane 2), 1:1000 (lane 3) or 1:2000 (lane 4).)

Related Product Information for anti-MKK4 antibody

The stress-activated protein kinases (SAPK) or Jun-amino-terminal kinases (JNK) are potently activated by stressors such as UV and gamma radiation. Similar to other MAP Kinases, the core signaling unit is composed of a MAPKKK, usually MEKK1-4 or a mixed lineage kinase (MLK), which phosphorylate and activate MKK4-7, leading to dual phosphorylation and activation of JNK kinases. The MKK family member MKK4 (SEK1/JNKK1) is activated downstream of Rho-GTPases by MEKK or MLK phosphorylation of Ser-257 and Thr-261, while Akt phosphorylation of Ser-80 inhibits MKK4 and suppresses stress-activated signal transduction.

References

Park, H. et al. (2002) J. Biol. Chem. 277:2573.
Kyriakis, J.M. et al. (1994) Nature 369:156.
Sanchez, I. et al. (1994) Nature 372:794.
Yan, M. et al. (1994) Nature 372:798.

NCBI and Uniprot Product Information

• NCBI GI #: 4506889
• NCBI GeneID: 6416
• NCBI Accession #: NP_003001.1
• NCBI GenBank Nucleotide #: NM_003010.2
• UniProt Accession #: P45985; Q5U0B8; Q6FHX4; Q6P9H2; Q6PIE6; B2R7N7; B3KYB2; D3DTS5
• Molecular Weight: 44,288 Da
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 4
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 4
• NCBI Official Symbol: MAP2K4
• NCBI Official Synonym Symbols: JNKK; MEK4; MKK4; SEK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 4; MEK 4; MAPKK 4; SAPK kinase 1; MAPK/ERK kinase 4; SAPK/ERK kinase 1; MAP kinase kinase 4; JNK-activated kinase 1; JNK activating kinase 1; JNK-activating kinase 1; c-Jun N-terminal kinase kinase
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 4
• Protein Family: Mitogen-activated protein kinase kinase
• UniProt Gene Name: MAP2K4
• UniProt Synonym Gene Names: JNKK1; MEK4; MKK4; PRKMK4; SEK1; SERK1; SKK1; MAP kinase kinase 4; MAPKK 4; MEK 4; SEK1; SAPK kinase 1; SAPKK-1; SAPKK1; JNKK
• UniProt Entry Name: MP2K4_HUMAN

NCBI Description

This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver. [provided by RefSeq, Jul 2008]

Uniprot Description

MKK4: dual specificity kinase of the STE7 family that phosphorylates and activates JNK1 and -2 as well as p38 but not ERK1 or -2. Mediates cellular responses to various cellular stresses and inflammatory cytokines. Phosphorylation by Akt inhibits MKK4 and suppresses stress-activated signal transduction.

Protein type: Kinase, protein; EC 2.7.12.2; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); STE group; STE7 family

Chromosomal Location of Human Ortholog: 17p12

Cellular Component: dendrite cytoplasm; axon; perikaryon; intracellular; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; mitogen-activated protein kinase kinase kinase binding; JUN kinase kinase activity; ATP binding; protein kinase activity

Biological Process: peptidyl-tyrosine phosphorylation; apoptosis; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; pathogenesis; toll-like receptor 3 signaling pathway; signal transduction; activation of JNK activity; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; positive regulation of neuron apoptosis; toll-like receptor signaling pathway; innate immune response; JNK cascade; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway; positive regulation of DNA replication; positive regulation of nitric-oxide synthase biosynthetic process