Interleukin 4 (IL4) Protein | IL4 protein

Eukaryotic Interleukin 4 (IL4)

Cat. Num. AAA2086681

• Gene Names: Il4; Il4e12
• Applications: SDS-Page, Western Blot
• Purity: > 95%
• Synonyms: Interleukin 4 (IL4); Eukaryotic Interleukin 4 (IL4); BSF1; BCGF1 ;B Cell Stimulatory Factor 1; Lymphocyte stimulatory factor 1; B Cell Growth Factor; Binetrakin; Pitrakinra; IL4 protein

• Host: 293F cell
• Purity/Purification: > 95%
• Form/Format: Freeze-dried powder
• Sequence Length: 147

• Applicable Applications for IL4 protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Fragment: Cys25~Ser147
• Tag: N-terminal His Tag
• Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
• Source Note: Eukaryotic expression
• Organism Species: Rat
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Isoelectric Point: 9.1
• Subcellular Location: Secreted
• Usage: Reconstitute in PBS or others.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

Product Categories/Family for IL4 protein

Eukaryotic proteins

NCBI and Uniprot Product Information

• NCBI GI #: 42627877
• NCBI GeneID: 287287
• NCBI Accession #: NP_958427.1
• NCBI GenBank Nucleotide #: NM_201270.1
• UniProt Accession #: P20096
• Molecular Weight: 15.4kDa; 18kDa
• NCBI Official Full Name: interleukin-4
• NCBI Official Synonym Full Names: interleukin 4
• NCBI Official Symbol: Il4
• NCBI Official Synonym Symbols: Il4e12
• NCBI Protein Information: interleukin-4
• UniProt Protein Name: Interleukin-4
• Protein Family: Interleukin
• UniProt Gene Name: Il4
• UniProt Synonym Gene Names: Il-4; IL-4; BSF-1

NCBI Description

Th2-type cytokine; may be involved in inflammatory response in eosinophils [RGD, Feb 2006]

Uniprot Description

IL4: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the IL-4/IL-13 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Cytokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q22

Cellular Component: external side of plasma membrane; extracellular space

Molecular Function: cytokine activity

Biological Process: B cell activation; B cell costimulation; cholesterol metabolic process; defense response to protozoan; female pregnancy; innate immune response in mucosa; microglial cell activation; myeloid dendritic cell differentiation; negative regulation of acute inflammatory response; negative regulation of chronic inflammatory response; negative regulation of macrophage activation; negative regulation of nitric oxide biosynthetic process; negative regulation of osteoclast differentiation; negative regulation of T cell activation; negative regulation of transcription, DNA-dependent; positive regulation of activated T cell proliferation; positive regulation of B cell activation; positive regulation of B cell proliferation; positive regulation of cell proliferation; positive regulation of chemokine biosynthetic process; positive regulation of defense response to virus by host; positive regulation of immunoglobulin production; positive regulation of interleukin-10 production; positive regulation of interleukin-13 production; positive regulation of isotype switching to IgE isotypes; positive regulation of isotype switching to IgG isotypes; positive regulation of mast cell degranulation; positive regulation of MHC class II biosynthetic process; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of T cell differentiation; positive regulation of T cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of tyrosine phosphorylation of Stat5 protein; regulation of immune response; regulation of inflammatory response; regulation of proton transport; regulation of T cell activation; response to cytokine stimulus; response to drug; response to ethanol; response to nutrient; response to organic cyclic substance; retina development in camera-type eye; T-helper 1 cell lineage commitment; T-helper 2 cell differentiation