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Western Blot (WB) (Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human MCF-7 Cells; Lane3: Human Jurkat Cells.)

Rabbit anti-Mouse Activating Transcription Factor 6 Polyclonal Antibody | anti-ATF6 antibody

Polyclonal Antibody to Activating Transcription Factor 6 (ATF6)

Gene Names
Atf6; ESTM49; AA789574; Atf6alpha; 9630036G24; 9130025P16Rik
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Activating Transcription Factor 6; Polyclonal Antibody; Polyclonal Antibody to Activating Transcription Factor 6 (ATF6); anti-ATF6 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against ATF6. It has beenselected for its ability to recognize ATF6 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
0.43mg/ml (varies by lot)
Sequence Length
670
Applicable Applications for anti-ATF6 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Fragment
ATF6 (Met1~Leu377)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Human, Mouse
Conjugate
No Conjugate
Immunogen
Recombinant ATF6 (Met1~Leu377) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2054712
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human MCF-7 Cells; Lane3: Human Jurkat Cells.)

Western Blot (WB) (Western Blot: Sample: Lane1: Human Hela Cells; Lane2: Human MCF-7 Cells; Lane3: Human Jurkat Cells.)

Western Blot (WB)

(Western Blot: Sample: Recombinant ATF6, Mouse.)

Western Blot (WB) (Western Blot: Sample: Recombinant ATF6, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Uterus Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Uterus Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Liver Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Liver Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Ductus Deferens Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Ductus Deferens Tissue)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
72,694 Da
NCBI Official Full Name
cyclic AMP-dependent transcription factor ATF-6 alpha
NCBI Official Synonym Full Names
activating transcription factor 6
NCBI Official Symbol
Atf6
NCBI Official Synonym Symbols
ESTM49; AA789574; Atf6alpha; 9630036G24; 9130025P16Rik
NCBI Protein Information
cyclic AMP-dependent transcription factor ATF-6 alpha
UniProt Protein Name
Cyclic AMP-dependent transcription factor ATF-6 alpha
UniProt Gene Name
Atf6
UniProt Synonym Gene Names
cAMP-dependent transcription factor ATF-6 alpha; ATF6-alpha

Uniprot Description

ATF6A: Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. Belongs to the bZIP family. ATF subfamily.

Protein type: Membrane protein, integral; Transcription factor

Chromosomal Location of Human Ortholog: 1 H3|1 76.96 cM

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; Golgi apparatus; integral component of endoplasmic reticulum membrane; integral component of membrane; membrane; nucleus

Molecular Function: DNA binding; DNA binding transcription factor activity; identical protein binding; protein binding; protein heterodimerization activity; sequence-specific DNA binding; ubiquitin protein ligase binding

Biological Process: endoplasmic reticulum unfolded protein response; eye development; positive regulation of apoptosis; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; response to unfolded protein; transcription, DNA-dependent; visual perception

Research Articles on ATF6

Similar Products

Product Notes

The ATF6 atf6 (Catalog #AAA2027740) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Activating Transcription Factor 6 (ATF6) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Activating Transcription Factor 6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the ATF6 atf6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Activating Transcription Factor 6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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