Rabbit PUMA Polyclonal Antibody | anti-PUMA antibody

PUMA (NT) Antibody

Cat. Num. AAA8002009

• Gene Names: BBC3; JFY1; PUMA; JFY-1
• Reactivity: Human, Rat, Fish
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A Purified
• Synonyms: PUMA; Polyclonal Antibody; PUMA (NT) Antibody; Rabbit Anti-Human PUMA (NT) Polyclonal; BBC3 Antibody; BCL2 binding component 3 Antibody; p53 up regulated modulator of apoptosis Antibody; PUMA/JFY1 Antibody; anti-PUMA antibody

• Host: Rabbit
• Reactivity: Human, Rat, Fish
• Clonality: Polyclonal
• Specificity: Detects the N-terminal domain of PUMA ~23kDa. Detects ~16kDa bands sometimes, possibly corresponding to PUMAbeta.
• Purity/Purification: Protein A Purified
• Form/Format: PBS, 0.02% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 193

• Applicable Applications for anti-PUMA antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500; IHC: 1:100
• Immunogen: N-terminal amino acids of human PUMA
• Immunogen Species: Human
• Conjugate: Unconjugated
• Cellular Localization: Mitochondrion
• Certificate of Analysis: 2 ug/ml of SPC-165 was sufficient for detection of PUMA in 20 ug of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Preparation and Storage: Store at-20 degree C.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-PUMA Polyclonal Antibody . Tissue: breast carcinoma. Species: Human. Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody at 1:100.)

Western Blot (WB)

(Western blot analysis of Human K562 cells lysates showing detection of PUMA protein using Rabbit Anti-PUMA Polyclonal Antibody . Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody at 1:500.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-PUMA Polyclonal Antibody . Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.)

Related Product Information for anti-PUMA antibody

Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Product Categories/Family for anti-PUMA antibody

Cancer; Apoptosis; Intracellular; Bcl2 Family; Cancer; Invasion/microenvironment; Cell Signaling; Metabolism; Mitochondrial Metabolism

References

1. Nakano K., Vousden K.H. (2001) Mol Cell. 2001; 7(3): 683-94. 2. Yu J., Zhang L., Hwang P.M., Kinzler K.W., Vogelstein B. (2001) Mol Cell. 7(3): 673-82. 3. Han J., et al. (2001) Proc Natl Acad Sci U S A. 98(20): 11318-23.

NCBI and Uniprot Product Information

• NCBI GI #: 56748610
• NCBI GeneID: 27113
• UniProt Accession #: Q9BXH1; Q96PG8
• NCBI Official Full Name: Bcl-2-binding component 3
• NCBI Official Synonym Full Names: BCL2 binding component 3
• NCBI Official Symbol: BBC3
• NCBI Official Synonym Symbols: JFY1; PUMA; JFY-1
• NCBI Protein Information: bcl-2-binding component 3
• UniProt Protein Name: Bcl-2-binding component 3
• UniProt Gene Name: BBC3
• UniProt Synonym Gene Names: PUMA
• UniProt Entry Name: BBC3_HUMAN

NCBI Description

This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]

Uniprot Description

PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol

Molecular Function: protein binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of protein homooligomerization; apoptosis; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of neuron apoptosis; release of sequestered calcium ion into cytosol; determination of adult life span; response to DNA damage stimulus; negative regulation of growth