Rabbit anti-Human PUMA (NT) Polyclonal Antibody | anti-BBC3 antibody

PUMA (NT) Antibody: ATTO 390

Cat. Num. AAA805513

• Gene Names: BBC3; JFY1; PUMA; JFY-1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Synonyms: PUMA (NT); Polyclonal Antibody; PUMA (NT) Antibody: ATTO 390; BBC3; BCL2 binding component 3; p53 up regulated modulator of apoptosis; PUMA/JFY1; anti-BBC3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Form/Format: Affinity Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 261

• Applicable Applications for anti-BBC3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: 1:500 (WB), 1:100 (IHC)
• Conjugate: ATTO 390
• Storage Buffer: PBS, 0.02% sodium azide
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-PUMA Polyclonal Antibody (SPC-165). Tissue: breast carcinoma. Species: Human. Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (SPC-165) at 1:100.)

Western Blot (WB)

(Western blot analysis of Human K562 cells lysates showing detection of PUMA protein using Rabbit Anti-PUMA Polyclonal Antibody (SPC-165). Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (SPC-165) at 1:500.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-PUMA Polyclonal Antibody (SPC-165). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody (SPC-165) at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.)

Related Product Information for anti-BBC3 antibody

Background Info: Detects a ~23kDa protein corresponding to the molecular mass of PUMA on SDS PAGE immunoblots. 16kDa bands may be seen in some instances, possibly corresponding to PUMAbeta.

Scientific Background: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Product Categories/Family for anti-BBC3 antibody

Apoptosis

References

1. Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell. 2001; 7(3):683-94. 2. Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol Cell. 2001;7(3):673-82. 3. Han J, Flemington C, Houghton AB, Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. Proc Natl Acad Sci U S A. 2001; 98(20):11318-23.

NCBI and Uniprot Product Information

• NCBI GI #: 187829730
• NCBI GeneID: 27113
• NCBI Accession #: NP_001120712.1
• NCBI GenBank Nucleotide #: NM_001127240.2
• UniProt Accession #: Q9BXH2; Q96PG8; Q9BXH1
• Molecular Weight: 20,532 Da
• NCBI Official Full Name: bcl-2-binding component 3 isoform 1
• NCBI Official Synonym Full Names: BCL2 binding component 3
• NCBI Official Symbol: BBC3
• NCBI Official Synonym Symbols: JFY1; PUMA; JFY-1
• NCBI Protein Information: bcl-2-binding component 3; p53 up-regulated modulator of apoptosis
• UniProt Protein Name: Bcl-2-binding component 3
• Protein Family: Bcl-2-binding component
• UniProt Gene Name: BBC3
• UniProt Synonym Gene Names: PUMA
• UniProt Entry Name: BBC3B_HUMAN