PUMA Recombinant Protein | BBC3 recombinant protein

PUMA Fragment Recombinant Protein

Cat. Num. AAA153295

• Gene Names: BBC3; JFY1; PUMA; JFY-1
• Applications: ELISA, Western Blot
• Purity: ~95%
• Synonyms: PUMA; PUMA Fragment Recombinant Protein; PUMA Antibody : JFY1; JFY-1; BCL2 binding component 3; BBC3 recombinant protein

• Purity/Purification: ~95%
• Form/Format: Liquid
• Sequence: aa. 76 - 170
• Sequence Length: 193

• Applicable Applications for BBC3 recombinant protein: ELISA (EIA), Western Blot (WB), MS
• Application Notes: This recombinant protein can be used for WB, ELISA, MS. For research use only.
• Source: E Coli
• Buffer: 1X PBS containing 0.1% SDS and 0.02% NaN₃
• Fusion Tag: Fusion Partner: His-tag and strepII-tag at N-terminus
• Preparation and Storage: Store in working aliquots at -70 degree C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

SDS-PAGE

(SDS-PAGE analysis of recombinant PUMA fragment on Coomassie Blue-stained 4-20% gradient gel.)

Related Product Information for BBC3 recombinant protein

Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-? and PUMA-? (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. This PUMA recombinant protein is recognized by several PUMA monoclonal antibodies, but not the two polyclonal antibodies (PSI # 3041 and 3043).

NCBI and Uniprot Product Information

• NCBI GI #: 56748610
• NCBI GeneID: 27113
• UniProt Accession #: Q9BXH1; O00171; Q96PG9; B9EGI3; Q96PG8
• Molecular Weight: 15 kDa (Calculated)
• NCBI Official Full Name: Bcl-2-binding component 3
• NCBI Official Synonym Full Names: BCL2 binding component 3
• NCBI Official Symbol: BBC3
• NCBI Official Synonym Symbols: JFY1; PUMA; JFY-1
• NCBI Protein Information: bcl-2-binding component 3; p53 up-regulated modulator of apoptosis
• UniProt Protein Name: Bcl-2-binding component 3
• Protein Family: Bcl-2-binding component
• UniProt Gene Name: BBC3
• UniProt Synonym Gene Names: PUMA
• UniProt Entry Name: BBC3_HUMAN

NCBI Description

This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]

Uniprot Description

PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol

Molecular Function: protein binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of protein homooligomerization; apoptosis; release of sequestered calcium ion into cytosol; positive regulation of neuron apoptosis; reduction of endoplasmic reticulum calcium ion concentration; determination of adult life span; response to DNA damage stimulus; negative regulation of growth