Porcine Retinoic acid induced protein 3 ELISA Kit | RIA3 elisa kit

Porcine Retinoic acid induced protein 3 ELISA Kit

Cat. Num. AAA745710

• Gene Names: GPRC5A; RAI3; RAIG1; GPCR5A
• Reactivity: Porcine
• Synonyms: Retinoic acid induced protein 3; Porcine Retinoic acid induced protein 3 ELISA Kit; RIA3 elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This RIA3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine RIA3. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RIA3 elisa kit

Principle of the assay: RIA3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RIA3 antibody and an RIA3-HRP conjugate. The assay sample and buffer are incubated together with RIA3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RIA3 concentration since RIA3 from samples and RIA3-HRP conjugate compete for the anti-RIA3 antibody binding site. Since the number of sites is limited, as more sites are occupied by RIA3 from the sample, fewer sites are left to bind RIA3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RIA3 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for RIA3 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4506403
• NCBI GeneID: 9052
• NCBI Accession #: NP_003970.1
• NCBI GenBank Nucleotide #: NM_003979.3
• Molecular Weight: 40,251 Da
• NCBI Official Full Name: retinoic acid-induced protein 3
• NCBI Official Synonym Full Names: G protein-coupled receptor, class C, group 5, member A
• NCBI Official Symbol: GPRC5A
• NCBI Official Synonym Symbols: RAI3; RAIG1; GPCR5A
• NCBI Protein Information: retinoic acid-induced protein 3; RAIG-1; retinoic acid induced 3; retinoic acid responsive; retinoic acid-induced gene 1 protein; orphan G-protein-coupling receptor PEIG-1; G-protein coupled receptor family C group 5 member A; G protein-coupled receptor, family C, group 5, member A
• UniProt Protein Name: Retinoic acid-induced protein 3
• UniProt Gene Name: GPRC5A
• UniProt Synonym Gene Names: GPCR5A; RAI3; RAIG1; RAIG-1
• UniProt Entry Name: RAI3_HUMAN

NCBI Description

This gene encodes a member of the type 3 G protein-coupling receptor family, characterized by the signature 7-transmembrane domain motif. The encoded protein may be involved in interaction between retinoid acid and G protein signalling pathways. Retinoic acid plays a critical role in development, cellular growth, and differentiation. This gene may play a role in embryonic development and epithelial cell differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

RAIG1: a G protein coupled receptor that is induced by all-trans retinoic acid (ATRA). Retinoic acids (RA) are a group of pleiotropic signaling molecules that regulate a broad range of physiologic and developmental processes. RAIG1 is expressed in lung tissue and in several lung cancer cell lines. This retinoic acid-inducible GPCR provides evidence for an interaction between retinoid and G-protein signaling pathways.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 3; Receptor, GPCR

Chromosomal Location of Human Ortholog: 12p13.1

Cellular Component: cytoplasmic vesicle membrane; intracellular membrane-bound organelle; integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; signal transduction