Rabbit anti-Human, Mouse PUMA Polyclonal Antibody | anti-PUMA antibody

PUMA (CT) Antibody

Cat. Num. AAA8002010

• Gene Names: BBC3; JFY1; PUMA; JFY-1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Protein A Purified
• Synonyms: PUMA; Polyclonal Antibody; PUMA (CT) Antibody; Rabbit Anti-Human PUMA (CT) Polyclonal; BBC3 Antibody; BCL2 binding component 3 Antibody; p53 up regulated modulator of apoptosis Antibody; PUMA/JFY1 Antibody; anti-PUMA antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Detects ~23kDa. Detects ~16kDa bands sometimes, possibly corresponding to PUMAbeta.
• Purity/Purification: Protein A Purified
• Form/Format: PBS, 0.02% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 261

• Applicable Applications for anti-PUMA antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:1000; ICC/IF: 1:100
• Immunogen: C-terminal amino acids of human PUMA
• Immunogen Species: Human
• Conjugate: Unconjugated
• Cellular Localization: Mitochondrion
• Certificate of Analysis: 1 ug/ml of SPC-166 was sufficient for detection of PUMA in 20 ug of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Preparation and Storage: Store at-20 degree C.

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PUMA (CT) Polyclonal Antibody . Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-PUMA (CT) Polyclonal Antibody at 1:125 for 12 hours at 4 degree C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-PUMA (CT) Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.)

Western Blot (WB)

(Western blot analysis of Human, Mouse K562 and 3T3 cell lysates showing detection of PUMA protein using Rabbit Anti-PUMA Polyclonal Antibody . Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody at 1:1000.)

Immunocytochemistry (ICC)/Immunofluorescence (IF)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PUMA (CT) Polyclonal Antibody . Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-PUMA (CT) Polyclonal Antibody at 1:125 for 12 hours at 4 degree C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-PUMA (CT) Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.)

Immunocytochemistry (ICC)/Immunofluorescence (IF)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PUMA Polyclonal Antibody . Tissue: K562 cells. Species: Human. Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody at 1:100.)

Related Product Information for anti-PUMA antibody

Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Product Categories/Family for anti-PUMA antibody

Cancer; Apoptosis; Intracellular; Bcl2 Family; Cancer; Invasion/microenvironment; Cell Signaling; Metabolism; Mitochondrial Metabolism

References

1. Nakano K., Vousden K.H. (2001) Mol Cell. 2001; 7(3): 683-94. 2. Yu J., Zhang L., Hwang P.M., Kinzler K.W., Vogelstein B. (2001) Mol Cell. 7(3): 673-82. 3. Han J., et al. (2001) Proc Natl Acad Sci U S A. 98(20): 11318-23.

NCBI and Uniprot Product Information

• NCBI GI #: 187829730
• NCBI GeneID: 27113
• NCBI Accession #: NP_001120712.1
• NCBI GenBank Nucleotide #: NM_001127240.2
• UniProt Accession #: Q9BXH3; Q96PG8; Q9BXH1
• NCBI Official Full Name: bcl-2-binding component 3 isoform 1
• NCBI Official Synonym Full Names: BCL2 binding component 3
• NCBI Official Symbol: BBC3
• NCBI Official Synonym Symbols: JFY1; PUMA; JFY-1
• NCBI Protein Information: bcl-2-binding component 3
• UniProt Protein Name: Bcl-2-binding component 3
• UniProt Gene Name: BBC3
• UniProt Synonym Gene Names: PUMA
• UniProt Entry Name: BBC3B_HUMAN

NCBI Description

This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]

Uniprot Description

CHST9: Catalyzes the transfer of sulfate to position 4 of non- reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. Participates in biosynthesis of glycoprotein hormones lutropin and thyrotropin, by mediating sulfation of their carbohydrate structures. Has a higher activity toward carbonic anhydrase VI than toward lutropin. Only active against terminal GalNAcbeta1,GalNAcbeta. Isoform 2, but not isoform 1, is active toward chondroitin. Belongs to the sulfotransferase 2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.8.2.-; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 18q11.2

Cellular Component: Golgi membrane; extracellular region; integral to membrane

Molecular Function: N-acetylgalactosamine 4-O-sulfotransferase activity

Biological Process: chondroitin sulfate metabolic process; sulfur metabolic process; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; proteoglycan biosynthetic process; carbohydrate metabolic process; pathogenesis; hormone biosynthetic process; carbohydrate biosynthetic process