Rabbit Proteasome alpha 5 Polyclonal Antibody | anti-psma5 antibody

Proteasome alpha 5 (PSMA5)

Cat. Num. AAA630537

• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Synonyms: Proteasome alpha 5; Polyclonal Antibody; Proteasome alpha 5 (PSMA5); Anti -Proteasome alpha 5 (PSMA5); anti-psma5 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of total human PSMA5 protein. Species crossreactivity: mouse, rat and monkey.
• Purity/Purification: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 0.15M sodium chloride, 0.1mg/ml BSA, 50% glycerol.

• Applicable Applications for anti-psma5 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Western Blot.; Dilution: Western Blot: 1:1000; Immunofluorescence (IF-IC): 1:50
• Immunogen: Synthetic peptide corresponding to residues surrounding Lys231 of human PSMA5 protein (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts from various cell lines using MBS630537. Lane 1: Molecular Weight markers, Lane 2: HEK293 cells, Lane 3: Neuro2A cells, Lane 4, PC12 cells, Lane 5: COS cells.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis HeLa cells using MBS630537 (green). Actin filaments have been labeled with Alexa Fluor ® 555 phalloidin (red).)

Related Product Information for anti-psma5 antibody

The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. It consists of four stacked rings, each with 7 distinct subunits. The two outer layers are identical rings composed of alpha subunits (called PSMAs), and the two inner layers are identical rings composed of beta subunits. While the catalytic sites are located on the beta rings (1-3), the alpha subunits are important for assembly and as binding sites for regulatory proteins (4). Seven different alpha and ten different beta proteasome genes have been identified in mammals (5). PA700, PA28 and PA200 are three major protein complexes that function as activators of the 20S proteasome. PA700 binds polyubiquitin with high affinity and associates with the 20S proteasome to form the 26S proteasome which preferentially degrades poly-ubiquitinylated proteins (1-3). The proteasome has a broad substrate spectrum which includes cell cycle regulators, signaling molecules, tumor suppressors and transcription factors. By controlling the degradation of these intracellular proteins, the proteasome functions in cell cycle regulation, cancer development, immune response, protein folding and disease progression (6-9).

Product Categories/Family for anti-psma5 antibody

Antibodies; Abs to Proteasomes

NCBI and Uniprot Product Information

• NCBI GI #: 148233324
• NCBI GeneID: 444215
• NCBI Accession #: NP_001085788.1
• NCBI GenBank Nucleotide #: NM_001092319.1
• NCBI Official Full Name: proteasome (prosome, macropain) subunit, alpha type, 5
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) subunit, alpha type, 5<
• NCBI Official Symbol: psma5
• NCBI Protein Information: proteasome (prosome, macropain) subunit, alpha type, 5; proteasome (prosome, macropain) subunit, alpha type, 5