Rabbit PSMA3 Polyclonal Antibody | anti-PSMA3 antibody

Anti-PSMA3 Antibody

Cat. Num. AAA419635

• Gene Names: PSMA3; HC8; PSC3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: PSMA3; Polyclonal Antibody; Anti-PSMA3 Antibody; anti-PSMA3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Western blot analysis of extracts of various cell lines, using PSMA3 antibody. Immunohistochemistry of paraffin-embedded rat lung using PSMA3 antibody at dilution of 1:200 (400x lens). Immunofluorescence analysis of MCF7 cell using PSMA3 antibody. Blue: DAPI for nuclear staining.
• Purity/Purification: Affinity Purification
• Form/Format: Liquid
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 248

• Applicable Applications for anti-PSMA3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50- 1:200; IF: 1:50- 1:200
• Immunogen: Recombinant protein of human PSMA3
• Storage Buffer: PBS with 0.02% sodium azide,50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

Immunohistochemistry (IHC)

Immunofluorescence (IF)

Related Product Information for anti-PSMA3 antibody

The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. It consists of four stacked rings, each with seven distinct subunits. The two outer layers are identical rings composed of +/- subunits (called PSMAs), and the two inner layers are identical rings composed of 2 subunits. While the catalytic sites are located on the 2 rings (1-3), the +/- subunits are important for assembly and as binding sites for regulatory proteins (4). Seven different +/- and ten different 2 proteasome genes have been identified in mammals (5). PA700, PA28, and PA200 are three major protein complexes that function as activators of the 20S proteasome. PA700 binds polyubiquitin with high affinity and associates with the 20S proteasome to form the 26S proteasome, which preferentially degrades poly-ubiquitinated proteins (1-3). The proteasome has a broad substrate spectrum that includes cell cycle regulators, signaling molecules, tumor suppressors, and transcription factors. By controlling the degradation of these intracellular proteins, the proteasome functions in cell cycle regulation, cancer development, immune responses, protein folding, and disease progression (6-9).

NCBI and Uniprot Product Information

• NCBI GI #: 23110939
• NCBI GeneID: 5684
• NCBI Accession #: NP_687033.1
• NCBI GenBank Nucleotide #: NM_152132.2
• UniProt Accession #: P25788; Q86U83; Q8N1D8; Q9BS70; B2RCK6
• Molecular Weight: 27,647 Da
• NCBI Official Full Name: proteasome subunit alpha type-3 isoform 2
• NCBI Official Synonym Full Names: proteasome subunit alpha 3
• NCBI Official Symbol: PSMA3
• NCBI Official Synonym Symbols: HC8; PSC3
• NCBI Protein Information: proteasome subunit alpha type-3
• UniProt Protein Name: Proteasome subunit alpha type-3
• Protein Family: Phenol-soluble modulin alpha 3 peptide
• UniProt Gene Name: PSMA3
• UniProt Synonym Gene Names: HC8; PSC8

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

PSMA3: a proteasomal protein of the T1A peptidase family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. Two alternatively spliced isoforms have been identified.

Protein type: EC 3.4.25.1; Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 14q23.1

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus; proteasome complex; proteasome core complex

Molecular Function: protein binding

Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; MAPKKK cascade; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; regulation of amino acid metabolic process; regulation of endopeptidase activity; regulation of mRNA stability; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; tumor necrosis factor-mediated signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway