Mouse L-amino-acid oxidase, IL4I1 ELISA Kit | IL4I1 elisa kit

Mouse L-amino-acid oxidase, IL4I1 ELISA Kit

Cat. Num. AAA1605213

• Reactivity: Mouse
• Synonyms: L-amino-acid oxidase; IL4I1; Mouse L-amino-acid oxidase; IL4I1 ELISA Kit; IL4I1 elisa kit

• Reactivity: Mouse
• Sequence Length: 517

• Samples: Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 10-2000ng/L
• Sensitivity: 5.62ng/L
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for IL4I1 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse L-amino-acid oxidase (also known as IL4I1) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse IL4I1 antibody. IL4I1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse IL4I1 Antibody is added and binds to IL4I1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL4I1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse IL4I1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 123913796
• UniProt Accession #: Q4JHE2
• NCBI Official Full Name: L-amino-acid oxidase
• UniProt Protein Name: L-amino-acid oxidase
• UniProt Gene Name: LAAO
• UniProt Synonym Gene Names: LAO

Uniprot Description

Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids, thus producing hydrogen peroxide that may contribute to the diverse toxic effects of this enzyme. Exhibits diverse biological activities, such as hemorrhage, hemolysis, edema, apoptosis of vascular endothelial cells or tumor cell lines, antibacterial and antiparasitic activities, as well as regulation of platelet aggregation. Effects of snake L-amino oxidases on platelets are controversial, since they either induce aggregation or inhibit agonist-induced aggregation. These different effects are probably due to different experimental conditions ().