Rabbit PSMA3 Polyclonal Antibody | anti-PSMA3 antibody

PSMA3 Polyclonal Antibody

Cat. Num. AAA127249

• Gene Names: PSMA3; HC8; PSC3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Affinity Purification
• Synonyms: PSMA3; Polyclonal Antibody; PSMA3 Polyclonal Antibody; HC8; MGC12306; MGC32631; PSC3; anti-PSMA3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Sequence Length: 255

• Applicable Applications for anti-PSMA3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IP: 1:50 - 1:100
• Species: Human
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human PSMA3 (NP_002779.1).
• Preparation and Storage: Store at -20°C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PSMA3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF-7 cells using PSMA3 antibody. Blue: DAPI for nuclear staining.)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of 200 ugextracts of HL-60 cells, using 3 ug PSMA3 antibody (MBS127249). Western blot was performed from the immunoprecipitate using PSMA3 antibody (MBS127249) at a dilition of 1:1000.)

Related Product Information for anti-PSMA3 antibody

The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. It consists of four stacked rings, each with seven distinct subunits. The two outer layers are identical rings composed of alpha subunits (called PSMAs), and the two inner layers are identical rings composed of beta subunits. While the catalytic sites are located on the beta rings (1-3), the alpha subunits are important for assembly and as binding sites for regulatory proteins (4). Seven different alpha and ten different beta proteasome genes have been identified in mammals (5). PA700, PA28, and PA200 are three major protein complexes that function as activators of the 20S proteasome. PA700 binds polyubiquitin with high affinity and associates with the 20S proteasome to form the 26S proteasome, which preferentially degrades poly-ubiquitinated proteins (1-3). The proteasome has a broad substrate spectrum that includes cell cycle regulators, signaling molecules, tumor suppressors, and transcription factors. By controlling the degradation of these intracellular proteins, the proteasome functions in cell cycle regulation, cancer development, immune responses, protein folding, and disease progression (6-9).

Product Categories/Family for anti-PSMA3 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 130859
• NCBI GeneID: 5684
• UniProt Accession #: P25788; Q86U83; Q8N1D8; Q9BS70; B2RCK6
• Molecular Weight: Calculated MW: 27kDa/28kDa; Observed MW: 28kDa
• NCBI Official Full Name: Proteasome subunit alpha type-3
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) subunit, alpha type, 3
• NCBI Official Symbol: PSMA3
• NCBI Official Synonym Symbols: HC8; PSC3
• NCBI Protein Information: proteasome subunit alpha type-3; macropain subunit C8; proteasome subunit C8; proteasome component C8; multicatalytic endopeptidase complex subunit C8
• UniProt Protein Name: Proteasome subunit alpha type-3
• Protein Family: Phenol-soluble modulin alpha 3 peptide
• UniProt Gene Name: PSMA3
• UniProt Synonym Gene Names: HC8; PSC8
• UniProt Entry Name: PSA3_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

PSMA3: a proteasomal protein of the T1A peptidase family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. Two alternatively spliced isoforms have been identified.

Protein type: Proteasome complex; Protease; EC 3.4.25.1

Chromosomal Location of Human Ortholog: 14q23

Cellular Component: nucleoplasm; proteasome complex; proteasome core complex; cytoplasm; cytosol; nucleus

Molecular Function: threonine endopeptidase activity; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle